Calcium Imaging of GPCR Activation Using Arrays of Reverse Transfected HEK293 Cells in a Microfluidic System

Calcium Imaging of GPCR Activation Using Arrays of Reverse Transfected HEK293 Cells in a Microfluidic System

Reverse-transfected cell arrays in microfluidic systems have great potential to perform large-scale parallel screening of G protein-coupled receptor (GPCR) activation. Here, we report the preparation of a novel platform using reverse transfection of HEK293 cells, imaging by stereo-fluorescence micro...

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Bibliographic Details
Main Authors: Margriet Roelse, Maurice G.L. Henquet, Harrie A. Verhoeven, Norbert C.A. de Ruijter, Ron Wehrens, Marco S. van Lenthe, Renger F. Witkamp, Robert D. Hall, Maarten A. Jongsma
Format: Article
Language:English
Published: MDPI AG 2018-02-01
Series:Sensors
Subjects:
reverse transfection
cell array
GPCR
NK1 receptor
Cameleon YC3.6
microfluidics
Online Access:http://www.mdpi.com/1424-8220/18/2/602

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