An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility
Objectives: To investigate the cause of an acute respiratory tract infection (ARTI) outbreak. Methods: Thirty-eight clinical samples were collected from 19 patients in an ARTI outbreak that occurred in a physical training facility in January 2013; patient demographic information was also collected....
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doaj-bc56d83ee9b044a094442683148cdee12020-11-24T23:09:59ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112014-11-0128C11712210.1016/j.ijid.2014.06.019An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facilityXiaoyan LiMei KongXu SuMing ZouLiru GuoXiaochun DongLin LiQing Gu Objectives: To investigate the cause of an acute respiratory tract infection (ARTI) outbreak. Methods: Thirty-eight clinical samples were collected from 19 patients in an ARTI outbreak that occurred in a physical training facility in January 2013; patient demographic information was also collected. In addition, 60 influenza virus-negative samples from febrile respiratory patients were collected from the same community at the same time to determine whether these were the same infections. Multiplex PCR (multi-PCR) was used to detect the possible pathogen in these samples. All human adenovirus (HAdV)-positive samples were inoculated onto Hep-2 cells for isolation. HAdV isolates were typed by hexon gene, fiber gene, and whole genome sequencing using primers designed in-house and compared to different type/serotype HAdVs downloaded from GenBank. Phylogenetic analysis was used to determine the type of the HAdV. Results: Of the 38 samples, 34 from 17 cases were HAdV-positive; two of them were co-infected, one with respiratory syncytial virus A and the other with human rhinovirus. The hexon gene open reading frame (ORF; 2841 nucleotides (nt)) and fiber gene ORF (978 nt) were obtained from four HAdV strains (TJ-2013-92, TJ-2013-94, TJ-2013-100, TJ-2013-122) from three upper respiratory infection cases and one pneumonia case. They were all completely identical. One HAdV isolate, TJ-2013-90, was selected for whole genome sequencing; 34 238 nt were obtained. Phylogenetic analysis showed the whole genome of TJ-2013-90 to be clustered together with HAdV-B55/HAdV-B11a. Three of 60 influenza virus-negative specimens were HAdV-positive, but hexon and fiber gene analysis showed that they were grouped in different branches to the HAdV isolates from this outbreak. Conclusions: The cause of this ARTI outbreak was HAdV-B55. This was another outbreak caused by this re-emerging virus. Continuous surveillance of respiratory adenovirus is necessary for disease control. http://www.sciencedirect.com/science/article/pii/S1201971214015811Human adenovirus-B55Respiratory infection outbreakWhole genome sequencePhylogenetic analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoyan Li Mei Kong Xu Su Ming Zou Liru Guo Xiaochun Dong Lin Li Qing Gu |
spellingShingle |
Xiaoyan Li Mei Kong Xu Su Ming Zou Liru Guo Xiaochun Dong Lin Li Qing Gu An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility International Journal of Infectious Diseases Human adenovirus-B55 Respiratory infection outbreak Whole genome sequence Phylogenetic analysis |
author_facet |
Xiaoyan Li Mei Kong Xu Su Ming Zou Liru Guo Xiaochun Dong Lin Li Qing Gu |
author_sort |
Xiaoyan Li |
title |
An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility |
title_short |
An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility |
title_full |
An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility |
title_fullStr |
An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility |
title_full_unstemmed |
An outbreak of acute respiratory disease in China caused by human adenovirus type B55 in a physical training facility |
title_sort |
outbreak of acute respiratory disease in china caused by human adenovirus type b55 in a physical training facility |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 1878-3511 |
publishDate |
2014-11-01 |
description |
Objectives: To investigate the cause of an acute respiratory tract infection (ARTI) outbreak.
Methods: Thirty-eight clinical samples were collected from 19 patients in an ARTI outbreak that occurred in a physical training facility in January 2013; patient demographic information was also collected. In addition, 60 influenza virus-negative samples from febrile respiratory patients were collected from the same community at the same time to determine whether these were the same infections. Multiplex PCR (multi-PCR) was used to detect the possible pathogen in these samples. All human adenovirus (HAdV)-positive samples were inoculated onto Hep-2 cells for isolation. HAdV isolates were typed by hexon gene, fiber gene, and whole genome sequencing using primers designed in-house and compared to different type/serotype HAdVs downloaded from GenBank. Phylogenetic analysis was used to determine the type of the HAdV.
Results: Of the 38 samples, 34 from 17 cases were HAdV-positive; two of them were co-infected, one with respiratory syncytial virus A and the other with human rhinovirus. The hexon gene open reading frame (ORF; 2841 nucleotides (nt)) and fiber gene ORF (978 nt) were obtained from four HAdV strains (TJ-2013-92, TJ-2013-94, TJ-2013-100, TJ-2013-122) from three upper respiratory infection cases and one pneumonia case. They were all completely identical. One HAdV isolate, TJ-2013-90, was selected for whole genome sequencing; 34 238 nt were obtained. Phylogenetic analysis showed the whole genome of TJ-2013-90 to be clustered together with HAdV-B55/HAdV-B11a. Three of 60 influenza virus-negative specimens were HAdV-positive, but hexon and fiber gene analysis showed that they were grouped in different branches to the HAdV isolates from this outbreak.
Conclusions: The cause of this ARTI outbreak was HAdV-B55. This was another outbreak caused by this re-emerging virus. Continuous surveillance of respiratory adenovirus is necessary for disease control.
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topic |
Human adenovirus-B55 Respiratory infection outbreak Whole genome sequence Phylogenetic analysis |
url |
http://www.sciencedirect.com/science/article/pii/S1201971214015811 |
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