Isolation and Identification of <i>Fusarium</i> spp., the Causal Agents of Onion (<i>Allium cepa</i>) Basal Rot in Northeastern Israel

• Main Authors: Ben Kalman, Dekel Abraham, Shaul Graph, Rafael Perl-Treves, Yael Meller Harel, Ofir Degani
• Format: Article
• Language: English
• Published: MDPI AG 2020-04-01
• Series: Biology
• Subjects: fungus; <i>Fusarium acutatum</i>; <i>Fusarium anthophilium</i>; <i>Fusarium oxysporum</i> f. sp. <i>cepae</i>; <i>Fusarium proliferatum</i>; molecular identification
• Online Access: https://www.mdpi.com/2079-7737/9/4/69

Over the past decade, there have been accumulating reports from farmers and field extension personnel on the increasing incidence and spread of onion (<i>Allium cepa</i>) bulb basal rot in northern Israel. The disease is caused mainly by <i>Fusarium</i> species. Rotting onion bulbs were sampled from fields in the Golan Heights in northeastern Israel during the summers of 2017 and 2018. Tissue from the sampled onion bulbs was used for the isolation and identification of the infecting fungal species using colony and microscopic morphology characterization. Final confirmation of the pathogens was performed with PCR amplification and sequencing using fungi-specific and <i>Fusarium</i> species-specific primers. Four <i>Fusarium</i> spp. isolates were identified in onion bulbs samples collected from the contaminated field: <i>F. proliferatum</i>, <i>F. oxysporum</i> f. sp. <i>cepae</i>, and two species less familiar as causative agents of this disease, <i>F. acutatum</i> and <i>F. anthophilium</i>. Phylogenetic analysis revealed that these species subdivided into two populations, a northern group isolated from white (Riverside cv.) onion bulbs, and a southern group isolated from red (565/505 cv.) bulbs. Pathogenicity tests conducted with seedlings and bulbs under moist conditions proved that all species could cause the disease symptoms, but with different degrees of virulence. Inoculating seeds with spore suspensions of the four species, <i>in vitro</i>, significantly reduced seedlings’ germination rate, hypocotyl elongation, and fresh biomass. Mature onion bulbs infected with the fungal isolates produced typical rot symptoms 14 days post-inoculation, and the fungus from each infected bulb was re-isolated and identified to satisfy Koch’s postulates. The onion bulb assay also reflected the degree of sensitivity of different onion cultivars to the disease. This work is the first confirmed report of the direct and primary cause of <i>Fusarium</i> onion basal rot disease in northeastern Israel. These findings are a necessary step towards uncovering the mycoflora of the diseased onion plants and developing a preventive program that would reduce the disease damage.