Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust
Thinopyrum ponticum (2n = 10x = 70) is a wild relative of wheat with high tolerance to both biotic and abiotic stresses; it has been wildly used in wheat genetic improvement. A disomic substitution line named SN19647 was derived from a cross between Triticum aestivum and the wheat–Th. ponticum parti...
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doaj-bc6d698233794c96855b5f307789b2862021-10-01T05:03:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.727734727734Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf RustMingzhu Li0Mingzhu Li1Yanzhen Wang2Xiaojuan Liu3Xiaojuan Liu4Xingfeng Li5Xingfeng Li6Honggang Wang7Honggang Wang8Yinguang Bao9Yinguang Bao10State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaAgronomy College of Shandong Agricultural University, Tai'an, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaAgronomy College of Shandong Agricultural University, Tai'an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaAgronomy College of Shandong Agricultural University, Tai'an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaAgronomy College of Shandong Agricultural University, Tai'an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, ChinaAgronomy College of Shandong Agricultural University, Tai'an, ChinaThinopyrum ponticum (2n = 10x = 70) is a wild relative of wheat with high tolerance to both biotic and abiotic stresses; it has been wildly used in wheat genetic improvement. A disomic substitution line named SN19647 was derived from a cross between Triticum aestivum and the wheat–Th. ponticum partial amphiploid SNTE20 (2n = 8x = 56). It was evaluated for disease resistance and characterized via sequential fluorescence in situ hybridization (FISH)-genomic in situ hybridization (GISH) and molecular markers. The results showed that SN19647 carried resistance to both powdery mildew and leaf rust. It contained 42 chromosomes with a pair of wheat chromosome 1B replaced by a pair of JS chromosomes from Th. ponticum. In addition to chromosomal substitution events, structural variation also occurred on wheat chromosomes 2A, 5A, 6B, and 7B. Based on marker analysis, 19 markers specific to the JS chromosome were obtained, of which seventeen markers belonged to homoeologous group one. These results indicated that SN19647 was a 1JS (1B) substitution line. Compared with the known 1JS (1D) substitution line CH10A5, it was found that 17 markers generated different specific bands to Th. ponticum, confirming the novelty of the 1JS chromosome in SN19647. Therefore, SN19647, resistant to powdery mildew and leaf rust, was a novel 1JS (1B) substitution line that can be used in wheat genetic improvement.https://www.frontiersin.org/articles/10.3389/fpls.2021.727734/fullThinopyrum ponticumsubstitution lineGISHFISHpowdery mildewleaf rust |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mingzhu Li Mingzhu Li Yanzhen Wang Xiaojuan Liu Xiaojuan Liu Xingfeng Li Xingfeng Li Honggang Wang Honggang Wang Yinguang Bao Yinguang Bao |
spellingShingle |
Mingzhu Li Mingzhu Li Yanzhen Wang Xiaojuan Liu Xiaojuan Liu Xingfeng Li Xingfeng Li Honggang Wang Honggang Wang Yinguang Bao Yinguang Bao Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust Frontiers in Plant Science Thinopyrum ponticum substitution line GISH FISH powdery mildew leaf rust |
author_facet |
Mingzhu Li Mingzhu Li Yanzhen Wang Xiaojuan Liu Xiaojuan Liu Xingfeng Li Xingfeng Li Honggang Wang Honggang Wang Yinguang Bao Yinguang Bao |
author_sort |
Mingzhu Li |
title |
Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust |
title_short |
Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust |
title_full |
Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust |
title_fullStr |
Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust |
title_full_unstemmed |
Molecular Cytogenetic Identification of a Novel Wheat–Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust |
title_sort |
molecular cytogenetic identification of a novel wheat–thinopyrum ponticum 1js (1b) substitution line resistant to powdery mildew and leaf rust |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-10-01 |
description |
Thinopyrum ponticum (2n = 10x = 70) is a wild relative of wheat with high tolerance to both biotic and abiotic stresses; it has been wildly used in wheat genetic improvement. A disomic substitution line named SN19647 was derived from a cross between Triticum aestivum and the wheat–Th. ponticum partial amphiploid SNTE20 (2n = 8x = 56). It was evaluated for disease resistance and characterized via sequential fluorescence in situ hybridization (FISH)-genomic in situ hybridization (GISH) and molecular markers. The results showed that SN19647 carried resistance to both powdery mildew and leaf rust. It contained 42 chromosomes with a pair of wheat chromosome 1B replaced by a pair of JS chromosomes from Th. ponticum. In addition to chromosomal substitution events, structural variation also occurred on wheat chromosomes 2A, 5A, 6B, and 7B. Based on marker analysis, 19 markers specific to the JS chromosome were obtained, of which seventeen markers belonged to homoeologous group one. These results indicated that SN19647 was a 1JS (1B) substitution line. Compared with the known 1JS (1D) substitution line CH10A5, it was found that 17 markers generated different specific bands to Th. ponticum, confirming the novelty of the 1JS chromosome in SN19647. Therefore, SN19647, resistant to powdery mildew and leaf rust, was a novel 1JS (1B) substitution line that can be used in wheat genetic improvement. |
topic |
Thinopyrum ponticum substitution line GISH FISH powdery mildew leaf rust |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.727734/full |
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