Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies
<i>Hedychium coronarium</i> J. Koenig is a multipurpose plant with significant economic value, but it has been overexploited and listed as a vulnerable, near threatened or endangered species. In vitro culture methods have been used for propagating disease-free propagules for its conserva...
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doaj-bc70e4c95e864fb79f05402502f490422021-04-02T15:48:51ZengMDPI AGAgronomy2073-43952020-07-01101068106810.3390/agronomy10081068Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like BodiesXiu Hu0Jiachuan Tan1Jianjun Chen2Yongquan Li3Jiaqi Huang4Department of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaDepartment of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaDepartment of Environmental Horticulture and Mid-Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Apopka, FL 32703, USADepartment of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaDepartment of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China<i>Hedychium coronarium</i> J. Koenig is a multipurpose plant with significant economic value, but it has been overexploited and listed as a vulnerable, near threatened or endangered species. In vitro culture methods have been used for propagating disease-free propagules for its conservation and production. However, explant contamination has been a bottleneck in in vitro propagation due to the use of rhizomes as the explant source. Plants in the family Zingiberaceae have pseudostems that support inflorescences, while rhizomes are considered true stems. The present study, for the first time, reported that the pseudostem bears nodes and vegetative buds and could actually be true stems. The evaluation of different sources of explants showed that mature node explants derived from the stem were the most suitable ones for in vitro culture because of the lowest contamination and the highest bud break rates. Culture of mature node explants on MS medium supplemented with 13.32, 17.76, and 22.20 μM 6-benzylaminopurine (BA), each in combination with 9.08 μM thidiazurin (TDZ) and 0.05 μM α-naphthaleneacetic acid (NAA) induced the conversion of buds to micro-rhizomes in six weeks. More than 96% of the micro-rhizomes cultured on MS medium supplemented with 17.76 μM BA, 6.81 μM TDZ, and 2.46 μM indole-3-butyric acid (IBA) were converted to globular-shaped clumps with protocorm-like bodies (PLBs). Further culture of a piece of the clumps induced more than 15 adventitious shoots. Adventitious roots were produced at the base of adventitious shoots, and plantlets were readily transplanted to a substrate for acclimatization in a shaded greenhouse. The survival rate of the plants in the greenhouse was up to 90%. Plants grew vigorously, and there were no off-types from the regenerated 11,100 plants. Our study also, for the first time, shows that <i>H. coronarium</i> can be regenerated via PLBs, which may represent a new way of the in vitro propagation of <i>H. coronarium</i>. The established protocol could be used for the increased propagation of <i>H. coronarium</i> for conservation or commercial production.https://www.mdpi.com/2073-4395/10/8/1068<i>Hedychium</i>medicinal plantsornamental plantsprotocorm-like bodies (PLBs)pseudostemrhizome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiu Hu Jiachuan Tan Jianjun Chen Yongquan Li Jiaqi Huang |
spellingShingle |
Xiu Hu Jiachuan Tan Jianjun Chen Yongquan Li Jiaqi Huang Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies Agronomy <i>Hedychium</i> medicinal plants ornamental plants protocorm-like bodies (PLBs) pseudostem rhizome |
author_facet |
Xiu Hu Jiachuan Tan Jianjun Chen Yongquan Li Jiaqi Huang |
author_sort |
Xiu Hu |
title |
Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies |
title_short |
Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies |
title_full |
Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies |
title_fullStr |
Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies |
title_full_unstemmed |
Efficient Regeneration of <i>Hedychium coronarium</i> through Protocorm-Like Bodies |
title_sort |
efficient regeneration of <i>hedychium coronarium</i> through protocorm-like bodies |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2020-07-01 |
description |
<i>Hedychium coronarium</i> J. Koenig is a multipurpose plant with significant economic value, but it has been overexploited and listed as a vulnerable, near threatened or endangered species. In vitro culture methods have been used for propagating disease-free propagules for its conservation and production. However, explant contamination has been a bottleneck in in vitro propagation due to the use of rhizomes as the explant source. Plants in the family Zingiberaceae have pseudostems that support inflorescences, while rhizomes are considered true stems. The present study, for the first time, reported that the pseudostem bears nodes and vegetative buds and could actually be true stems. The evaluation of different sources of explants showed that mature node explants derived from the stem were the most suitable ones for in vitro culture because of the lowest contamination and the highest bud break rates. Culture of mature node explants on MS medium supplemented with 13.32, 17.76, and 22.20 μM 6-benzylaminopurine (BA), each in combination with 9.08 μM thidiazurin (TDZ) and 0.05 μM α-naphthaleneacetic acid (NAA) induced the conversion of buds to micro-rhizomes in six weeks. More than 96% of the micro-rhizomes cultured on MS medium supplemented with 17.76 μM BA, 6.81 μM TDZ, and 2.46 μM indole-3-butyric acid (IBA) were converted to globular-shaped clumps with protocorm-like bodies (PLBs). Further culture of a piece of the clumps induced more than 15 adventitious shoots. Adventitious roots were produced at the base of adventitious shoots, and plantlets were readily transplanted to a substrate for acclimatization in a shaded greenhouse. The survival rate of the plants in the greenhouse was up to 90%. Plants grew vigorously, and there were no off-types from the regenerated 11,100 plants. Our study also, for the first time, shows that <i>H. coronarium</i> can be regenerated via PLBs, which may represent a new way of the in vitro propagation of <i>H. coronarium</i>. The established protocol could be used for the increased propagation of <i>H. coronarium</i> for conservation or commercial production. |
topic |
<i>Hedychium</i> medicinal plants ornamental plants protocorm-like bodies (PLBs) pseudostem rhizome |
url |
https://www.mdpi.com/2073-4395/10/8/1068 |
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