Cloning and Expression of a Novel Leucine Dehydrogenase: Characterization and L-tert-Leucine Production
Among many genes encoding for amino acid dehydrogenase, a novel leucine dehydrogenase gene from Exiguobacterium sibiricum (EsiLeuDH) was isolated by using genome mining strategy. EsiLeuDH was overexpressed in Escherichia coli BL21 (DE3), followed by purification and characterization. The high thermo...
Main Authors: | Wei Luo, Jing Zhu, Yuzheng Zhao, Huili Zhang, Xue Yang, Yuantao Liu, Zhiming Rao, Xiaobin Yu |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-03-01
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Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00186/full |
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