Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CH...

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Main Authors: Stefanie Schuster, Srinethe Saravanakumar, Ludger Schöls, Stefan Hauser
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118303131
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spelling doaj-bc79e48a9faa4ccf95efb529db870ded2020-11-24T21:38:49ZengElsevierStem Cell Research1873-50612019-01-0134Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locusStefanie Schuster0Srinethe Saravanakumar1Ludger Schöls2Stefan Hauser3Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Graduate School of Cellular and Molecular Neuroscience, University of Tübingen, Tübingen, GermanyHertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, GermanyHertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, GermanyGerman Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Corresponding author.STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency.http://www.sciencedirect.com/science/article/pii/S1873506118303131
collection DOAJ
language English
format Article
sources DOAJ
author Stefanie Schuster
Srinethe Saravanakumar
Ludger Schöls
Stefan Hauser
spellingShingle Stefanie Schuster
Srinethe Saravanakumar
Ludger Schöls
Stefan Hauser
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
Stem Cell Research
author_facet Stefanie Schuster
Srinethe Saravanakumar
Ludger Schöls
Stefan Hauser
author_sort Stefanie Schuster
title Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
title_short Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
title_full Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
title_fullStr Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
title_full_unstemmed Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
title_sort generation of a homozygous crispr/cas9-mediated knockout human ipsc line for the stub1 locus
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2019-01-01
description STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency.
url http://www.sciencedirect.com/science/article/pii/S1873506118303131
work_keys_str_mv AT stefanieschuster generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus
AT srinethesaravanakumar generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus
AT ludgerschols generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus
AT stefanhauser generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus
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