Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CH...
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doaj-bc79e48a9faa4ccf95efb529db870ded2020-11-24T21:38:49ZengElsevierStem Cell Research1873-50612019-01-0134Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locusStefanie Schuster0Srinethe Saravanakumar1Ludger Schöls2Stefan Hauser3Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Graduate School of Cellular and Molecular Neuroscience, University of Tübingen, Tübingen, GermanyHertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, GermanyHertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, GermanyGerman Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Corresponding author.STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency.http://www.sciencedirect.com/science/article/pii/S1873506118303131 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefanie Schuster Srinethe Saravanakumar Ludger Schöls Stefan Hauser |
spellingShingle |
Stefanie Schuster Srinethe Saravanakumar Ludger Schöls Stefan Hauser Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus Stem Cell Research |
author_facet |
Stefanie Schuster Srinethe Saravanakumar Ludger Schöls Stefan Hauser |
author_sort |
Stefanie Schuster |
title |
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus |
title_short |
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus |
title_full |
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus |
title_fullStr |
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus |
title_full_unstemmed |
Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus |
title_sort |
generation of a homozygous crispr/cas9-mediated knockout human ipsc line for the stub1 locus |
publisher |
Elsevier |
series |
Stem Cell Research |
issn |
1873-5061 |
publishDate |
2019-01-01 |
description |
STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency. |
url |
http://www.sciencedirect.com/science/article/pii/S1873506118303131 |
work_keys_str_mv |
AT stefanieschuster generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus AT srinethesaravanakumar generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus AT ludgerschols generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus AT stefanhauser generationofahomozygouscrisprcas9mediatedknockouthumanipsclineforthestub1locus |
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