Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos)
H5N1 influenza represents one of the great challenges to public health. Some H5N1 viruses (i.e., A/goose/Hubei/65/05, GS/65) are weakly pathogenic, while the others (i.e., A/duck/Hubei/49/05, DK/49) are highly pathogenic to their natural hosts. Here, we performed brain and spleen transcriptomic anal...
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doaj-bc9708bea19c4338916c07d033d53a4e2021-06-08T04:41:16ZengElsevierJournal of Integrative Agriculture2095-31192019-07-0118714601472Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos)Yin-hua HUANG0Hua-peng FENG1Li-ren HUANG2Kang YI3En-guang RONG4Xiao-yun CHEN5Jian-wen LI6Zeng WANG7Peng-yang ZHU8Xiao-juan LIU9Xiao-xue WANG10Jia-xiang HU11Xin LIU12Hua-lan CHEN13Jun WANG14Ning LI15State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China; Correspondence HUANG Yin-huaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaBGI-Shenzhen, Shenzhen 518083, P.R.ChinaState Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.ChinaChina Veterinary Culture Collection Center, China Institute of Veterinary Drug Control, Beijing 100081, P.R.ChinaBGI-Shenzhen, Shenzhen 518083, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaState Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.ChinaState Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.ChinaState Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.ChinaBGI-Shenzhen, Shenzhen 518083, P.R.ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R.ChinaBGI-Shenzhen, Shenzhen 518083, P.R.ChinaState Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.ChinaH5N1 influenza represents one of the great challenges to public health. Some H5N1 viruses (i.e., A/goose/Hubei/65/05, GS/65) are weakly pathogenic, while the others (i.e., A/duck/Hubei/49/05, DK/49) are highly pathogenic to their natural hosts. Here, we performed brain and spleen transcriptomic analyses of control ducks and ones infected by the DK/49 or the GS/65 H5N1 virus. We demonstrated that, compared to the GS/65 virus, the DK/49 virus infection changed more numerous immune genes’ expression and caused continuous increasing of immune pathways (i.e., RIG-I and MDA5) in ducks. We found that both H5N1 virus strains might escape or subvert host immune response through affecting alternative translation of immune genes, while the DK/49 virus seemed to induce alternative translation of more immune genes than the GS/65 virus. We also identified five co-expressional modules associated with H5N1 virus replication through the weight correlation network analysis (WGCNA). Moreover, we first demonstrated that the duck BCL2L15 and DCSTAMP in one of these five modules inhibited both the highly pathogenic and weakly pathogenic H5N1 virus replication efficiently. These analyses, in combination with our comprehensive transcriptomic data, provided global view of the molecular architecture for the interaction between host and H5N1 viruses.http://www.sciencedirect.com/science/article/pii/S2095311919626468duckinnate immune genesH5N1 influenza virusestranscriptomes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin-hua HUANG Hua-peng FENG Li-ren HUANG Kang YI En-guang RONG Xiao-yun CHEN Jian-wen LI Zeng WANG Peng-yang ZHU Xiao-juan LIU Xiao-xue WANG Jia-xiang HU Xin LIU Hua-lan CHEN Jun WANG Ning LI |
spellingShingle |
Yin-hua HUANG Hua-peng FENG Li-ren HUANG Kang YI En-guang RONG Xiao-yun CHEN Jian-wen LI Zeng WANG Peng-yang ZHU Xiao-juan LIU Xiao-xue WANG Jia-xiang HU Xin LIU Hua-lan CHEN Jun WANG Ning LI Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos) Journal of Integrative Agriculture duck innate immune genes H5N1 influenza viruses transcriptomes |
author_facet |
Yin-hua HUANG Hua-peng FENG Li-ren HUANG Kang YI En-guang RONG Xiao-yun CHEN Jian-wen LI Zeng WANG Peng-yang ZHU Xiao-juan LIU Xiao-xue WANG Jia-xiang HU Xin LIU Hua-lan CHEN Jun WANG Ning LI |
author_sort |
Yin-hua HUANG |
title |
Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos) |
title_short |
Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos) |
title_full |
Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos) |
title_fullStr |
Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos) |
title_full_unstemmed |
Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck (Anas platyrhynchos) |
title_sort |
transcriptomic analyses reveal new genes and networks response to h5n1 influenza viruses in duck (anas platyrhynchos) |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2019-07-01 |
description |
H5N1 influenza represents one of the great challenges to public health. Some H5N1 viruses (i.e., A/goose/Hubei/65/05, GS/65) are weakly pathogenic, while the others (i.e., A/duck/Hubei/49/05, DK/49) are highly pathogenic to their natural hosts. Here, we performed brain and spleen transcriptomic analyses of control ducks and ones infected by the DK/49 or the GS/65 H5N1 virus. We demonstrated that, compared to the GS/65 virus, the DK/49 virus infection changed more numerous immune genes’ expression and caused continuous increasing of immune pathways (i.e., RIG-I and MDA5) in ducks. We found that both H5N1 virus strains might escape or subvert host immune response through affecting alternative translation of immune genes, while the DK/49 virus seemed to induce alternative translation of more immune genes than the GS/65 virus. We also identified five co-expressional modules associated with H5N1 virus replication through the weight correlation network analysis (WGCNA). Moreover, we first demonstrated that the duck BCL2L15 and DCSTAMP in one of these five modules inhibited both the highly pathogenic and weakly pathogenic H5N1 virus replication efficiently. These analyses, in combination with our comprehensive transcriptomic data, provided global view of the molecular architecture for the interaction between host and H5N1 viruses. |
topic |
duck innate immune genes H5N1 influenza viruses transcriptomes |
url |
http://www.sciencedirect.com/science/article/pii/S2095311919626468 |
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