Neuroprotective Activity of Triterpenoid Saponins from Platycodi radix Against Glutamate-induced Toxicity in Primary Cultured Rat Cortical Cells

• Main Authors: Hyung-In Moon, Hyun Duk Yang, Sung Ik Lee, Yong Hoon Park, Il Hong Son
• Format: Article
• Language: English
• Published: MDPI AG 2007-05-01
• Series: Molecules
• Subjects: Platycodi radix; neuroprotective; triterpenes saponin; glutamate-induced toxicity
• Online Access: http://www.mdpi.com/1420-3049/12/5/1147/

During our investigation of the neuroprotective activity of Platycodi radix we found that an aqueous extract of this folk medicine exhibited significant protection against glutamate-induced toxicity in primary cultured rat cortical cells. In order to clarify the neuroprotective mechanism(s) of this observed effect, activity-guided isolation was performed to seek and identify active fractions and components. By such fractionation, four known triterpene saponin compounds - platycodins A, C and D and deapioplatycodin D - were isolated from the n-butanol fraction. Among these four compounds, platycodin A exhibited significant neuroprotective activities against glutamate-induced toxicity, exhibiting cell viability of about 50 %, at concentrations ranging from 0.1 μM to 10 μM. Therefore, the neuroprotective effect of Platycodi radix might be due to the inhibition of glutamate-induced toxicity by the saponin compounds it contains.