Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production
The polyketide, 6-pentyl-alpha-pyrone (6-PP), has a characteristic coconut aroma and is produced by many Trichoderma species. In this study, we screened formerly isolated Trichoderma strains for 6-PP production grown using solid-state fermentation (SSF). Sugarcane bagasse supplemented with a nutrien...
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doaj-bc9f832f3e444fe19c6fc9036036b2132020-11-25T00:16:15ZengHindawi LimitedJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/85623848562384Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia ProductionRayhane Hamrouni0Josiane Molinet1Lucie Miché2Quentin Carboué3Nathalie Dupuy4Ahmed Masmoudi5Sevastianos Roussos6Aix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, FranceAix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, FranceAix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, FranceAix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, FranceAix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, FranceUniv. Manouba, ISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, 2020 Ariana, TunisiaAix Marseille Univ, Avignon Université, CNRS, IRD, IMBE, Marseille, FranceThe polyketide, 6-pentyl-alpha-pyrone (6-PP), has a characteristic coconut aroma and is produced by many Trichoderma species. In this study, we screened formerly isolated Trichoderma strains for 6-PP production grown using solid-state fermentation (SSF). Sugarcane bagasse supplemented with a nutrient solution was used as the support material for the culture. We detected outstandingly strong 6-PP production in the case of two Trichoderma strains in this experiment. The strains were identified based on internal transcribed spacer (ITS) sequence analysis as Trichoderma asperellum (G7 and G18). We also investigated the production of 6-PP in the case of the best Trichoderma strain selected (T. asperellum G7) from two types of cultivation (SSF and liquid cultivation (LC)). The same liquid medium was used to impregnate sugarcane bagasse. Results led to a 6-PP production 80 times higher (85.11 μg·g·DM−1) in SSF than that found in LC (10.00 μg·L−1) after eight days of cultivation. For this, the SSF is the best option to produce 6-PP compared to liquid cultures.http://dx.doi.org/10.1155/2019/8562384 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rayhane Hamrouni Josiane Molinet Lucie Miché Quentin Carboué Nathalie Dupuy Ahmed Masmoudi Sevastianos Roussos |
spellingShingle |
Rayhane Hamrouni Josiane Molinet Lucie Miché Quentin Carboué Nathalie Dupuy Ahmed Masmoudi Sevastianos Roussos Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production Journal of Chemistry |
author_facet |
Rayhane Hamrouni Josiane Molinet Lucie Miché Quentin Carboué Nathalie Dupuy Ahmed Masmoudi Sevastianos Roussos |
author_sort |
Rayhane Hamrouni |
title |
Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production |
title_short |
Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production |
title_full |
Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production |
title_fullStr |
Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production |
title_full_unstemmed |
Production of Coconut Aroma in Solid-State Cultivation: Screening and Identification of Trichoderma Strains for 6-Pentyl-Alpha-Pyrone and Conidia Production |
title_sort |
production of coconut aroma in solid-state cultivation: screening and identification of trichoderma strains for 6-pentyl-alpha-pyrone and conidia production |
publisher |
Hindawi Limited |
series |
Journal of Chemistry |
issn |
2090-9063 2090-9071 |
publishDate |
2019-01-01 |
description |
The polyketide, 6-pentyl-alpha-pyrone (6-PP), has a characteristic coconut aroma and is produced by many Trichoderma species. In this study, we screened formerly isolated Trichoderma strains for 6-PP production grown using solid-state fermentation (SSF). Sugarcane bagasse supplemented with a nutrient solution was used as the support material for the culture. We detected outstandingly strong 6-PP production in the case of two Trichoderma strains in this experiment. The strains were identified based on internal transcribed spacer (ITS) sequence analysis as Trichoderma asperellum (G7 and G18). We also investigated the production of 6-PP in the case of the best Trichoderma strain selected (T. asperellum G7) from two types of cultivation (SSF and liquid cultivation (LC)). The same liquid medium was used to impregnate sugarcane bagasse. Results led to a 6-PP production 80 times higher (85.11 μg·g·DM−1) in SSF than that found in LC (10.00 μg·L−1) after eight days of cultivation. For this, the SSF is the best option to produce 6-PP compared to liquid cultures. |
url |
http://dx.doi.org/10.1155/2019/8562384 |
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