Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study
Alterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. A new imaging technique—named dynamic nuclear polarization (DNP) carbon-13 magnetic resonance imaging (MRI)—probes the glycolytic flux in a real-time, dynamic...
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doaj-bcac068a856b456692c21f5f7351b89c2021-08-26T14:03:48ZengMDPI AGMetabolites2218-19892021-08-011151851810.3390/metabo11080518Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging StudyYing-Chieh Lai0Ching-Yi Hsieh1Kuan-Ying Lu2Cheng-Hsuan Sung3Hung-Yao Ho4Mei-Ling Cheng5Albert P. Chen6Shu-Hang Ng7Fang-Hsin Chen8Gigin Lin9Department of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanClinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanDepartment of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanMedical Imaging Research Center, Institute for Radiological Research, Chang Gung University, Taoyuan 333, TaiwanClinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanClinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanGE Healthcare, Toronto, ON M5V 3Y3, CanadaDepartment of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanDepartment of Medical Imaging and Radiological Sciences, Chang Gung University, Taoyuan 333, TaiwanDepartment of Medical Imaging and Intervention, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanAlterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. A new imaging technique—named dynamic nuclear polarization (DNP) carbon-13 magnetic resonance imaging (MRI)—probes the glycolytic flux in a real-time, dynamic manner. The [1-<sup>13</sup>C]pyruvate is transported by the monocarboxylate transporter (MCT) into cells and converted into [1-<sup>13</sup>C]lactate by lactate dehydrogenase (LDH). To capture the early glycolytic alterations in the irradiated cancer and immune cells, we designed a preliminary DNP <sup>13</sup>C-MRI study by using hyperpolarized [1-<sup>13</sup>C]pyruvate to study human FaDu squamous carcinoma cells, HMC3 microglial cells, and THP-1 monocytes before and after irradiation. The pyruvate-to-lactate conversion rate (<i>k</i><sub>PL</sub> [Pyr.]) calculated by kinetic modeling was used to evaluate the metabolic alterations. Western blotting was performed to assess the expressions of LDHA, LDHB, MCT1, and MCT4 proteins. Following irradiation, the pyruvate-to-lactate conversion rates on DNP <sup>13</sup>C-MRI were significantly decreased in the FaDu and the HMC3 cells but increased in the THP-1 cells. Western blot analysis confirmed the similar trends in LDHA and LDHB expression levels. In conclusion, DNP <sup>13</sup>C-MRI non-invasively captured the different glycolytic alterations among cancer and immune systems in response to irradiation, implying its potential for clinical use in the future.https://www.mdpi.com/2218-1989/11/8/518cancer metabolismdynamic nuclear polarizationglycolysisimmune systemmagnetic resonance imagingradiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying-Chieh Lai Ching-Yi Hsieh Kuan-Ying Lu Cheng-Hsuan Sung Hung-Yao Ho Mei-Ling Cheng Albert P. Chen Shu-Hang Ng Fang-Hsin Chen Gigin Lin |
spellingShingle |
Ying-Chieh Lai Ching-Yi Hsieh Kuan-Ying Lu Cheng-Hsuan Sung Hung-Yao Ho Mei-Ling Cheng Albert P. Chen Shu-Hang Ng Fang-Hsin Chen Gigin Lin Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites cancer metabolism dynamic nuclear polarization glycolysis immune system magnetic resonance imaging radiation |
author_facet |
Ying-Chieh Lai Ching-Yi Hsieh Kuan-Ying Lu Cheng-Hsuan Sung Hung-Yao Ho Mei-Ling Cheng Albert P. Chen Shu-Hang Ng Fang-Hsin Chen Gigin Lin |
author_sort |
Ying-Chieh Lai |
title |
Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study |
title_short |
Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study |
title_full |
Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study |
title_fullStr |
Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study |
title_full_unstemmed |
Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study |
title_sort |
monitoring early glycolytic flux alterations following radiotherapy in cancer and immune cells: hyperpolarized carbon-13 magnetic resonance imaging study |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2021-08-01 |
description |
Alterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. A new imaging technique—named dynamic nuclear polarization (DNP) carbon-13 magnetic resonance imaging (MRI)—probes the glycolytic flux in a real-time, dynamic manner. The [1-<sup>13</sup>C]pyruvate is transported by the monocarboxylate transporter (MCT) into cells and converted into [1-<sup>13</sup>C]lactate by lactate dehydrogenase (LDH). To capture the early glycolytic alterations in the irradiated cancer and immune cells, we designed a preliminary DNP <sup>13</sup>C-MRI study by using hyperpolarized [1-<sup>13</sup>C]pyruvate to study human FaDu squamous carcinoma cells, HMC3 microglial cells, and THP-1 monocytes before and after irradiation. The pyruvate-to-lactate conversion rate (<i>k</i><sub>PL</sub> [Pyr.]) calculated by kinetic modeling was used to evaluate the metabolic alterations. Western blotting was performed to assess the expressions of LDHA, LDHB, MCT1, and MCT4 proteins. Following irradiation, the pyruvate-to-lactate conversion rates on DNP <sup>13</sup>C-MRI were significantly decreased in the FaDu and the HMC3 cells but increased in the THP-1 cells. Western blot analysis confirmed the similar trends in LDHA and LDHB expression levels. In conclusion, DNP <sup>13</sup>C-MRI non-invasively captured the different glycolytic alterations among cancer and immune systems in response to irradiation, implying its potential for clinical use in the future. |
topic |
cancer metabolism dynamic nuclear polarization glycolysis immune system magnetic resonance imaging radiation |
url |
https://www.mdpi.com/2218-1989/11/8/518 |
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