Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses
In the present study, long-term organic and conventional managements were compared at the experimental field of Monsampolo del Tronto (Marche region, Italy) with the aim of investigating soil chemical fertility and microbial community structure. A polyphasic approach, combining soil fertility indica...
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doaj-bcb3d56298a74ad68b4467c3a7f240252020-11-24T23:13:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-12-01510.3389/fmicb.2014.00644119397Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analysesFabio eStagnari0Giorgia ePerpetuini1Rosanna eTofalo2Gabriele eCampanelli3Fabrizio eLeteo4Umberto eDella vella5Maria eSchirone6Giovanna eSuzzi7Michele ePisante8University of TeramoUniversity of TeramoUniversity of TeramoConsiglio per la ricerca e la sperimentazione in Agricoltura - Unità di ricerca per l'orticoltura (CRA - ORA) Consiglio per la ricerca e la sperimentazione in Agricoltura - Unità di ricerca per l'orticoltura (CRA - ORA) University of TeramoUniversity of TeramoUniversity of TeramoUniversity of TeramoIn the present study, long-term organic and conventional managements were compared at the experimental field of Monsampolo del Tronto (Marche region, Italy) with the aim of investigating soil chemical fertility and microbial community structure. A polyphasic approach, combining soil fertility indicators with microbiological analyses (plate counts, PCR-denaturing gradient gel electrophoresis [DGGE] and phospholipid fatty acid analysis [PLFA]) was applied. Organic matter, N as well as some important macro and micronutrients (K, P, Mg, Mn, Cu and Zn) for crop growth, were more available under organic management. Bacterial counts were higher in organic management. A significant influence of management system and management x crop interaction was observed for total mesophilic bacteria, nitrogen fixing bacteria and actinobacteria. Interestingly, cultivable fungi were not detected in all analyzed samples. PLFA biomass was higher in the organic and Gram positive bacteria dominated the microbial community in both systems. Even if fungal biomass was higher in organic management, fungal PCR-DGGE fingerprinting revealed that the two systems were very similar in terms of fungal species suggesting that 10 years were not enough to establish a new dynamic equilibrium among ecosystem components. A better knowledge of soil biota and in particular of fungal community structure will be useful for the development of sustainable management strategies.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00644/fullMicrobial groupsorganic and conventional foodfungal communitysoil fertility indicatorslong- term soil management |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fabio eStagnari Giorgia ePerpetuini Rosanna eTofalo Gabriele eCampanelli Fabrizio eLeteo Umberto eDella vella Maria eSchirone Giovanna eSuzzi Michele ePisante |
spellingShingle |
Fabio eStagnari Giorgia ePerpetuini Rosanna eTofalo Gabriele eCampanelli Fabrizio eLeteo Umberto eDella vella Maria eSchirone Giovanna eSuzzi Michele ePisante Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses Frontiers in Microbiology Microbial groups organic and conventional food fungal community soil fertility indicators long- term soil management |
author_facet |
Fabio eStagnari Giorgia ePerpetuini Rosanna eTofalo Gabriele eCampanelli Fabrizio eLeteo Umberto eDella vella Maria eSchirone Giovanna eSuzzi Michele ePisante |
author_sort |
Fabio eStagnari |
title |
Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses |
title_short |
Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses |
title_full |
Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses |
title_fullStr |
Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses |
title_full_unstemmed |
Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses |
title_sort |
long-term impact of farm management and crops on soil microorganisms assessed by combined dgge and plfa analyses |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2014-12-01 |
description |
In the present study, long-term organic and conventional managements were compared at the experimental field of Monsampolo del Tronto (Marche region, Italy) with the aim of investigating soil chemical fertility and microbial community structure. A polyphasic approach, combining soil fertility indicators with microbiological analyses (plate counts, PCR-denaturing gradient gel electrophoresis [DGGE] and phospholipid fatty acid analysis [PLFA]) was applied. Organic matter, N as well as some important macro and micronutrients (K, P, Mg, Mn, Cu and Zn) for crop growth, were more available under organic management. Bacterial counts were higher in organic management. A significant influence of management system and management x crop interaction was observed for total mesophilic bacteria, nitrogen fixing bacteria and actinobacteria. Interestingly, cultivable fungi were not detected in all analyzed samples. PLFA biomass was higher in the organic and Gram positive bacteria dominated the microbial community in both systems. Even if fungal biomass was higher in organic management, fungal PCR-DGGE fingerprinting revealed that the two systems were very similar in terms of fungal species suggesting that 10 years were not enough to establish a new dynamic equilibrium among ecosystem components. A better knowledge of soil biota and in particular of fungal community structure will be useful for the development of sustainable management strategies. |
topic |
Microbial groups organic and conventional food fungal community soil fertility indicators long- term soil management |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00644/full |
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