The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell
The current work investigated the α-amylase inhibitory activity of five selected Pinto bean peptides (PBPs) using AR42J cell. The objectives were: (a) to prove the potential of PBPs as α-amylase inhibitor in living cells, and (b) to investigate the inhibition mode of PBPs. Results showed that PBP9 o...
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doaj-bcc46225c87b428cbbbac781d26ea74e2021-04-30T07:10:54ZengElsevierJournal of Functional Foods1756-46462017-08-0135641647The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cellYing-Yuan Ngoh0Gee Jun Tye1Chee-Yuen Gan2Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 Penang, MalaysiaInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, MalaysiaAnalytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 Penang, Malaysia; Corresponding author at: Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.The current work investigated the α-amylase inhibitory activity of five selected Pinto bean peptides (PBPs) using AR42J cell. The objectives were: (a) to prove the potential of PBPs as α-amylase inhibitor in living cells, and (b) to investigate the inhibition mode of PBPs. Results showed that PBP9 obtained the lowest IC50 value of 0.31 mM followed by PBP7 (5.92 mM), PBP1 (6.08 mM), PBP6 (6.14 mM) and PBP3 (6.64 mM). In the MTT assay, all PBP showed a dose-dependent trend, whereby lower concentration of PBP demonstrated a higher cell viability. By using PBP concentrations of 0.33–8.3 mM, Michaelis-Menten kinetic parameters and Lineweaver-Burk plots revealed that Km and Vmax values were ranged from 38.52–794.38 mM and 0.72–1.15 mM/min, respectively, resulting in uncompetitive (PBP3, PBP6 and PBP7) and unusual (PBP1 and PBP9) α-amylase inhibition modes. It was suggested that PBP could be considered as new agents for the diabetes treatment.http://www.sciencedirect.com/science/article/pii/S1756464617303559α-AmylaseAR42J cellDiabetes treatmentInhibitory activityPinto bean peptide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying-Yuan Ngoh Gee Jun Tye Chee-Yuen Gan |
spellingShingle |
Ying-Yuan Ngoh Gee Jun Tye Chee-Yuen Gan The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell Journal of Functional Foods α-Amylase AR42J cell Diabetes treatment Inhibitory activity Pinto bean peptide |
author_facet |
Ying-Yuan Ngoh Gee Jun Tye Chee-Yuen Gan |
author_sort |
Ying-Yuan Ngoh |
title |
The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell |
title_short |
The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell |
title_full |
The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell |
title_fullStr |
The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell |
title_full_unstemmed |
The investigation of α-amylase inhibitory activity of selected Pinto bean peptides via preclinical study using AR42J cell |
title_sort |
investigation of α-amylase inhibitory activity of selected pinto bean peptides via preclinical study using ar42j cell |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2017-08-01 |
description |
The current work investigated the α-amylase inhibitory activity of five selected Pinto bean peptides (PBPs) using AR42J cell. The objectives were: (a) to prove the potential of PBPs as α-amylase inhibitor in living cells, and (b) to investigate the inhibition mode of PBPs. Results showed that PBP9 obtained the lowest IC50 value of 0.31 mM followed by PBP7 (5.92 mM), PBP1 (6.08 mM), PBP6 (6.14 mM) and PBP3 (6.64 mM). In the MTT assay, all PBP showed a dose-dependent trend, whereby lower concentration of PBP demonstrated a higher cell viability. By using PBP concentrations of 0.33–8.3 mM, Michaelis-Menten kinetic parameters and Lineweaver-Burk plots revealed that Km and Vmax values were ranged from 38.52–794.38 mM and 0.72–1.15 mM/min, respectively, resulting in uncompetitive (PBP3, PBP6 and PBP7) and unusual (PBP1 and PBP9) α-amylase inhibition modes. It was suggested that PBP could be considered as new agents for the diabetes treatment. |
topic |
α-Amylase AR42J cell Diabetes treatment Inhibitory activity Pinto bean peptide |
url |
http://www.sciencedirect.com/science/article/pii/S1756464617303559 |
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