Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters
To obtain an accurate picture of microbial processes is essential for in-depth understanding of the dynamics of microbial communities and metabolic potentials. In this study, an apparatus for automatic multiple in situ nucleic acid collections (MISNAC) loaded and controlled by a lander was designed...
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2020-02-01
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doaj-bcdf937c6a0848b49610c0ec2ae51a722020-11-25T02:21:35ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-02-01710.3389/fmars.2020.00081490618Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea WatersZhan-Fei Wei0Zhan-Fei Wei1Wen-Li Li2Jun Li3Jun Chen4Yong-Zhi Xin5Li-Sheng He6Yong Wang7Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaCollege of Earth Sciences, University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaInstitute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, ChinaTo obtain an accurate picture of microbial processes is essential for in-depth understanding of the dynamics of microbial communities and metabolic potentials. In this study, an apparatus for automatic multiple in situ nucleic acid collections (MISNAC) loaded and controlled by a lander was designed and applied for in situ microbial filtration, cell lysis and nucleic acid collection at different time points during three cruises in the South China Sea. The MISNAC apparatus completed 390 L in situ water filtration and collected a total of 1,176 ng DNA with six working units in four deployments at ∼1,000 m depth. Microbial community structures in water samples obtained by the MISNAC and in situ microbial filtration and fixation (ISMIFF) apparatus, respectively, were compared by analyzing sequences of 16S rRNA amplicons. The result showed that these communities were largely consistent, regardless of the differences in the communities in the samples from different cruises and time points. In addition, the transcriptomes of the samples collected by the MISANC and a Niskin bottle dramatically differed in abundance and diversity of 16S rRNA gene reads, indicating that more in situ RNA molecules were preserved by the MISNAC, compared to the Niskin. The application of the MISNAC apparatus paved the way to time-series changes of deep-sea microorganisms and their response to various environmental factors.https://www.frontiersin.org/article/10.3389/fmars.2020.00081/fullin situ filtrationDNA extractioncell lysisdeep-seamicrobial community |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhan-Fei Wei Zhan-Fei Wei Wen-Li Li Jun Li Jun Chen Yong-Zhi Xin Li-Sheng He Yong Wang |
spellingShingle |
Zhan-Fei Wei Zhan-Fei Wei Wen-Li Li Jun Li Jun Chen Yong-Zhi Xin Li-Sheng He Yong Wang Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters Frontiers in Marine Science in situ filtration DNA extraction cell lysis deep-sea microbial community |
author_facet |
Zhan-Fei Wei Zhan-Fei Wei Wen-Li Li Jun Li Jun Chen Yong-Zhi Xin Li-Sheng He Yong Wang |
author_sort |
Zhan-Fei Wei |
title |
Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters |
title_short |
Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters |
title_full |
Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters |
title_fullStr |
Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters |
title_full_unstemmed |
Multiple in situ Nucleic Acid Collections (MISNAC) From Deep-Sea Waters |
title_sort |
multiple in situ nucleic acid collections (misnac) from deep-sea waters |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Marine Science |
issn |
2296-7745 |
publishDate |
2020-02-01 |
description |
To obtain an accurate picture of microbial processes is essential for in-depth understanding of the dynamics of microbial communities and metabolic potentials. In this study, an apparatus for automatic multiple in situ nucleic acid collections (MISNAC) loaded and controlled by a lander was designed and applied for in situ microbial filtration, cell lysis and nucleic acid collection at different time points during three cruises in the South China Sea. The MISNAC apparatus completed 390 L in situ water filtration and collected a total of 1,176 ng DNA with six working units in four deployments at ∼1,000 m depth. Microbial community structures in water samples obtained by the MISNAC and in situ microbial filtration and fixation (ISMIFF) apparatus, respectively, were compared by analyzing sequences of 16S rRNA amplicons. The result showed that these communities were largely consistent, regardless of the differences in the communities in the samples from different cruises and time points. In addition, the transcriptomes of the samples collected by the MISANC and a Niskin bottle dramatically differed in abundance and diversity of 16S rRNA gene reads, indicating that more in situ RNA molecules were preserved by the MISNAC, compared to the Niskin. The application of the MISNAC apparatus paved the way to time-series changes of deep-sea microorganisms and their response to various environmental factors. |
topic |
in situ filtration DNA extraction cell lysis deep-sea microbial community |
url |
https://www.frontiersin.org/article/10.3389/fmars.2020.00081/full |
work_keys_str_mv |
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