Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
Abstract Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA...
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doaj-bce1a1b8bf434bd4a1df0871f8556f482021-09-19T11:29:13ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111610.1038/s41598-021-97753-yExtracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAsElisangela de Paula Silva0Luciana Cavalheiro Marti1Flávia Maziero Andreghetto2Romário Oliveira de Sales3Martin Hoberman4Bárbara dos Santos Dias5Larissa Figueiredo Alves Diniz6Alessandro Marins dos Santos7Raquel Ajub Moyses8Otávio Alberto Curioni9Rossana Veronica Mendoza Lopez10Fabio Daumas Nunes11Eloiza Helena Tajara12Patricia Severino13Centro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinHead and Neck Surgery Department, Hospital das Clínicas da Faculdade de Medicina, Universidade de São PauloDepartamento de Cirurgia de Cabeça e Pescoço e Otorrinolaringologia, Hospital HeliópolisCentro de Investigação Translacional em Oncologia, Instituto do Câncer do Estado de São PauloDepartment of Oral Pathology, School of Dentistry, Universidade de São PauloDepartment of Molecular Biology, Faculdade de Medicina de São José Do Rio PretoCentro de Pesquisa Experimental, Albert Einstein Research and Education Institute, Hospital Israelita Albert EinsteinAbstract Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling of EVs released from two distinct cell lines and treated dendritic cells derived from circulating monocytes (mono-DCs) with these EVs. We confirmed the internalization of EVs by mono-DCs and the down-regulation of microRNA mRNA targets in treated mono-DCs. Differences in surface markers of dendritic cells cultivated in the presence of EVs indicated that their content disrupts the maturation process. Additionally, microRNAs known to interfere with dendritic cell function, and detected in EVs, matched microRNAs from squamous cell carcinoma patients’ plasma: miR-17-5p in oropharyngeal squamous cell carcinoma, miR-21 in oral squamous cell carcinoma, miR-16, miR-24, and miR-181a circulating in both oral and oropharyngeal squamous cell carcinoma, and miR-23b, which has not been previously described in plasma of head and neck squamous cell carcinoma, was found in plasma from patients with these cancer subtypes. This study contributes with insights on EVs in signaling between cancer and immune cells in squamous cell carcinoma of the head and neck.https://doi.org/10.1038/s41598-021-97753-y |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Elisangela de Paula Silva Luciana Cavalheiro Marti Flávia Maziero Andreghetto Romário Oliveira de Sales Martin Hoberman Bárbara dos Santos Dias Larissa Figueiredo Alves Diniz Alessandro Marins dos Santos Raquel Ajub Moyses Otávio Alberto Curioni Rossana Veronica Mendoza Lopez Fabio Daumas Nunes Eloiza Helena Tajara Patricia Severino |
spellingShingle |
Elisangela de Paula Silva Luciana Cavalheiro Marti Flávia Maziero Andreghetto Romário Oliveira de Sales Martin Hoberman Bárbara dos Santos Dias Larissa Figueiredo Alves Diniz Alessandro Marins dos Santos Raquel Ajub Moyses Otávio Alberto Curioni Rossana Veronica Mendoza Lopez Fabio Daumas Nunes Eloiza Helena Tajara Patricia Severino Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs Scientific Reports |
author_facet |
Elisangela de Paula Silva Luciana Cavalheiro Marti Flávia Maziero Andreghetto Romário Oliveira de Sales Martin Hoberman Bárbara dos Santos Dias Larissa Figueiredo Alves Diniz Alessandro Marins dos Santos Raquel Ajub Moyses Otávio Alberto Curioni Rossana Veronica Mendoza Lopez Fabio Daumas Nunes Eloiza Helena Tajara Patricia Severino |
author_sort |
Elisangela de Paula Silva |
title |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
title_short |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
title_full |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
title_fullStr |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
title_full_unstemmed |
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs |
title_sort |
extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma micrornas |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-09-01 |
description |
Abstract Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling of EVs released from two distinct cell lines and treated dendritic cells derived from circulating monocytes (mono-DCs) with these EVs. We confirmed the internalization of EVs by mono-DCs and the down-regulation of microRNA mRNA targets in treated mono-DCs. Differences in surface markers of dendritic cells cultivated in the presence of EVs indicated that their content disrupts the maturation process. Additionally, microRNAs known to interfere with dendritic cell function, and detected in EVs, matched microRNAs from squamous cell carcinoma patients’ plasma: miR-17-5p in oropharyngeal squamous cell carcinoma, miR-21 in oral squamous cell carcinoma, miR-16, miR-24, and miR-181a circulating in both oral and oropharyngeal squamous cell carcinoma, and miR-23b, which has not been previously described in plasma of head and neck squamous cell carcinoma, was found in plasma from patients with these cancer subtypes. This study contributes with insights on EVs in signaling between cancer and immune cells in squamous cell carcinoma of the head and neck. |
url |
https://doi.org/10.1038/s41598-021-97753-y |
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