A new 6-hydroxy-4-sphingenine-containing ceramide in human skin
A new ceramide consisting of 6-hydroxysphingosine linked to a non-hydroxyacid was found in human epidermal lipid. This ceramide was sought because its fatty acid and sphingoid moieties are present in other combinations in human epidermal ceramides. To isolate the new ceramide, the mixture of ceramid...
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1999-08-01
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doaj-bce67c2f85ca4181a6fbd8daf7d117fb2021-04-27T04:44:50ZengElsevierJournal of Lipid Research0022-22751999-08-0140814341439A new 6-hydroxy-4-sphingenine-containing ceramide in human skinMary Ellen Stewart0Donald Talbot Downing1To whom correspondence should be addressed.; The Marshall Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City, IA 52242The Marshall Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City, IA 52242A new ceramide consisting of 6-hydroxysphingosine linked to a non-hydroxyacid was found in human epidermal lipid. This ceramide was sought because its fatty acid and sphingoid moieties are present in other combinations in human epidermal ceramides. To isolate the new ceramide, the mixture of ceramides in human epidermal lipid was first separated into fractions by thin-layer chromatography (TLC), and then each fraction was further purified by TLC after acetylation of all hydroxyl groups. TLC after acetylation revealed that one of the fractions isolated in the first TLC step contained two components, namely, the ceramide consisting of sphingosine linked to an α-hydroxyacid and an unknown ceramide. The new ceramide constituted about 9% of the total ceramides, and was shown by NMR spectroscopy to be N-acyl-6-hydroxysphingosine.— Stewart, M. E., and D. T. Downing. A new 6-hydroxy-4-sphingenine-containing ceramide in human skin. J. Lipid Res. 1999. 40: 1434–1439.http://www.sciencedirect.com/science/article/pii/S002222752033385XNMRα-hydroxyacidsepidermal lipids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mary Ellen Stewart Donald Talbot Downing |
spellingShingle |
Mary Ellen Stewart Donald Talbot Downing A new 6-hydroxy-4-sphingenine-containing ceramide in human skin Journal of Lipid Research NMR α-hydroxyacids epidermal lipids |
author_facet |
Mary Ellen Stewart Donald Talbot Downing |
author_sort |
Mary Ellen Stewart |
title |
A new 6-hydroxy-4-sphingenine-containing ceramide in human skin |
title_short |
A new 6-hydroxy-4-sphingenine-containing ceramide in human skin |
title_full |
A new 6-hydroxy-4-sphingenine-containing ceramide in human skin |
title_fullStr |
A new 6-hydroxy-4-sphingenine-containing ceramide in human skin |
title_full_unstemmed |
A new 6-hydroxy-4-sphingenine-containing ceramide in human skin |
title_sort |
new 6-hydroxy-4-sphingenine-containing ceramide in human skin |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1999-08-01 |
description |
A new ceramide consisting of 6-hydroxysphingosine linked to a non-hydroxyacid was found in human epidermal lipid. This ceramide was sought because its fatty acid and sphingoid moieties are present in other combinations in human epidermal ceramides. To isolate the new ceramide, the mixture of ceramides in human epidermal lipid was first separated into fractions by thin-layer chromatography (TLC), and then each fraction was further purified by TLC after acetylation of all hydroxyl groups. TLC after acetylation revealed that one of the fractions isolated in the first TLC step contained two components, namely, the ceramide consisting of sphingosine linked to an α-hydroxyacid and an unknown ceramide. The new ceramide constituted about 9% of the total ceramides, and was shown by NMR spectroscopy to be N-acyl-6-hydroxysphingosine.— Stewart, M. E., and D. T. Downing. A new 6-hydroxy-4-sphingenine-containing ceramide in human skin. J. Lipid Res. 1999. 40: 1434–1439. |
topic |
NMR α-hydroxyacids epidermal lipids |
url |
http://www.sciencedirect.com/science/article/pii/S002222752033385X |
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