A proteomic platform to identify off-target proteins associated with therapeutic modalities that induce protein degradation or gene silencing
Abstract Novel modalities such as PROTAC and RNAi have the ability to inadvertently alter the abundance of endogenous proteins. Currently available in vitro secondary pharmacology assays, which evaluate off-target binding or activity of small molecules, do not fully assess the off-target effects of...
Main Authors: | Xin Liu, Ye Zhang, Lucas D. Ward, Qinghong Yan, Tanggis Bohnuud, Rocio Hernandez, Socheata Lao, Jing Yuan, Fan Fan |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-95354-3 |
Similar Items
-
Evidence of conformational selection driving the formation of ligand binding sites in protein-protein interfaces.
by: Tanggis Bohnuud, et al.
Published: (2014-10-01) -
Targeted Protein Degradation: Unlocking the Therapeutic Proteome
by: EBioMedicine
Published: (2018-04-01) -
Silencing sounds off
by: Yu-Fan Chen, et al.
Published: (2015-03-01) -
Optical Detection of Degraded Therapeutic Proteins
by: Wu, Di, et al.
Published: (2018) -
Off-line LC-MALDI techniques for the identification of proteins, post-translational modifications and protein-protein interactions in proteomic studies
by: Chen, Vincent C.
Published: (2013)