| Summary: | An ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-methylquinoxaline-2-carboxylic acid) and aminoglycoside antibiotics(Tilmicosin, Tylosin) residues in Penaeus. vannamei, Samples were extracted with acidified acetonitrile, then cleaned up with hexane. The separation of targets was carried out on a Waters BEH C18 column using acetonitrile and 0. 1% fomicacid as mobile phase. Analyte identification and quantification were performed by MS/MS under multiple-reaction monitering(MRM) mode with the external standard method. The results showed that there were good linear relationships between peak area and concentrations of nine targets in the range of 2.5 ~ 50 μg/L, with correlation coefficients more than 0. 999 and The limits of detection(LOD) were in the range of 0. 1 ~ 0.9 μg/kg. the recoveries ranged from 80.2% ~ 94.0%, with RSDs of 1.7%~8.4%. The method is simple, accurate and precise, and It could satisfy the requirement of mass and fast analysis in the laborator.
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