Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry
An ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-...
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2020-01-01
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doaj-bcee7b6fca5c440c83865da39ee1a3762021-04-02T19:03:13ZengEDP SciencesE3S Web of Conferences2267-12422020-01-012130103310.1051/e3sconf/202021301033e3sconf_acic2020_01033Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass SpectrometryZha Yu-bing0Yang Chun-liangLv Yi-zhi1Ye Jian-zhiZeng Shao-dongWang Xiao-fang2Deng Xiao-hong3Ma Hui-fangPan XiaoweiLin Li-yunAgriculture Products Processing Research Institute of South ChinaAgricultural technology extension CenterAgriculture Products Processing Research Institute of South ChinaAgricultural technology extension CenterAn ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-methylquinoxaline-2-carboxylic acid) and aminoglycoside antibiotics(Tilmicosin, Tylosin) residues in Penaeus. vannamei, Samples were extracted with acidified acetonitrile, then cleaned up with hexane. The separation of targets was carried out on a Waters BEH C18 column using acetonitrile and 0. 1% fomicacid as mobile phase. Analyte identification and quantification were performed by MS/MS under multiple-reaction monitering(MRM) mode with the external standard method. The results showed that there were good linear relationships between peak area and concentrations of nine targets in the range of 2.5 ~ 50 μg/L, with correlation coefficients more than 0. 999 and The limits of detection(LOD) were in the range of 0. 1 ~ 0.9 μg/kg. the recoveries ranged from 80.2% ~ 94.0%, with RSDs of 1.7%~8.4%. The method is simple, accurate and precise, and It could satisfy the requirement of mass and fast analysis in the laborator.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/73/e3sconf_acic2020_01033.pdf |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zha Yu-bing Yang Chun-liang Lv Yi-zhi Ye Jian-zhi Zeng Shao-dong Wang Xiao-fang Deng Xiao-hong Ma Hui-fang Pan Xiaowei Lin Li-yun |
spellingShingle |
Zha Yu-bing Yang Chun-liang Lv Yi-zhi Ye Jian-zhi Zeng Shao-dong Wang Xiao-fang Deng Xiao-hong Ma Hui-fang Pan Xiaowei Lin Li-yun Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry E3S Web of Conferences |
author_facet |
Zha Yu-bing Yang Chun-liang Lv Yi-zhi Ye Jian-zhi Zeng Shao-dong Wang Xiao-fang Deng Xiao-hong Ma Hui-fang Pan Xiaowei Lin Li-yun |
author_sort |
Zha Yu-bing |
title |
Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry |
title_short |
Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry |
title_full |
Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry |
title_fullStr |
Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry |
title_full_unstemmed |
Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry |
title_sort |
determination of residues of two aminoglycoside antibiotics and five quinololine antibiotics and their metabolites in penaeus vannamei by ultra high performance liquid chromatography tandem mass spectrometry |
publisher |
EDP Sciences |
series |
E3S Web of Conferences |
issn |
2267-1242 |
publishDate |
2020-01-01 |
description |
An ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-methylquinoxaline-2-carboxylic acid) and aminoglycoside antibiotics(Tilmicosin, Tylosin) residues in Penaeus. vannamei, Samples were extracted with acidified acetonitrile, then cleaned up with hexane. The separation of targets was carried out on a Waters BEH C18 column using acetonitrile and 0. 1% fomicacid as mobile phase. Analyte identification and quantification were performed by MS/MS under multiple-reaction monitering(MRM) mode with the external standard method. The results showed that there were good linear relationships between peak area and concentrations of nine targets in the range of 2.5 ~ 50 μg/L, with correlation coefficients more than 0. 999 and The limits of detection(LOD) were in the range of 0. 1 ~ 0.9 μg/kg. the recoveries ranged from 80.2% ~ 94.0%, with RSDs of 1.7%~8.4%. The method is simple, accurate and precise, and It could satisfy the requirement of mass and fast analysis in the laborator. |
url |
https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/73/e3sconf_acic2020_01033.pdf |
work_keys_str_mv |
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