The recovery effect of neuregulin on the sciatic nerve constriction injury as well as its molecular mechanism

• Main Authors: Xin-Jun Wu, Fei Xiang, Hong Mu
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-04-01
• Series: Journal of Hainan Medical University
• Subjects: Neuregulin Sciatic nerve constriction Fibrosis PI3K Akt
• Online Access: http://www.hnykdxxb.com/PDF/201708/4.pdf
• ISSN: 1007-1237; 1007-1237

Objective: To study the recovery effect of neuregulin (Nrg-1) on the sciatic nerve constriction injury as well as the expression of Nrg-1 downstream signaling molecules in sciatic nerve tissue. Methods: SD rats were selected as the experimental animals and divided into sham operation + saline intervention group (group A), sciatic nerve constriction model + saline intervention group (group B) and sciatic nerve constriction model + Nrg-1 intervention group (group C). 2 weeks, 4 weeks and 8 weeks after intervention, the sciatic nerve function parameters were detected; 8 weeks after intervention, the levels of fibrosis molecules and Nrg-1 downstream molecules in sciatic nerve tissue were determined. Results: 2 weeks, 4 weeks and 8 weeks after intervention, sciatic nerve MCV and CMAP amplitudes of group B were significantly lower than those of group A, and the sciatic nerve MCV and CMAP amplitudes of group C were significantly higher than those of group B; 8 weeks after intervention, CTGF, Col-I, Col-III, MMP2, MMP9 and PTEN levels in sciatic nerve tissue of group B were significantly higher than those of group A while ErbB, Akt and PI3K levels were significantly lower than those of group A; CTGF, Col-I, Col-III, MMP2, MMP9 and PTEN levels in sciatic nerve tissue of group C were significantly lower than those of group B while ErbB, Akt and PI3K levels were significantly higher than those of group B. Conclusion: Neuregulin can activate the PI3K/Akt to exert the recovery effect on sciatic nerve constriction injury.