Red-mediated transposition and final release of the mini-F vector of a cloned infectious herpesvirus genome.
Bacterial artificial chromosomes (BACs) are well-established cloning vehicles for functional genomics and for constructing targeting vectors and infectious viral DNA clones. Red-recombination-based mutagenesis techniques have enabled the manipulation of BACs in Escherichia coli without any remaining...
Main Authors: | Felix Wussow, Helmut Fickenscher, B Karsten Tischer |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2009-12-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2780728?pdf=render |
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