The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i>
White spot syndrome virus (WSSV), the lone virus of the genus <i>Whispovirus</i> under the family <i>Nimaviridae</i>, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limit...
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doaj-bd1862c84e9748c9b2aa1e533ff334fd2020-11-25T02:13:03ZengMDPI AGGenes2073-44252020-01-011119410.3390/genes11010094genes11010094The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i>Weidong Bao0Kathy F. J. Tang1Acacia Alcivar-Warren2Genetic Information Research Institute, 20380 Town Center Lane, Suite 240, Cupertino, CA 95014, USAYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaFundación para la Conservation de la Biodiversidad Acuática y Terrestre (FUCOBI), Quito EC1701, EcuadorWhite spot syndrome virus (WSSV), the lone virus of the genus <i>Whispovirus</i> under the family <i>Nimaviridae</i>, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limited, partly due to its large genome and the lack of other closely related free-living viruses for comparative studies. In this study, we reconstructed a full-length endogenous nimavirus consensus genome, <i>Nimav-1_LVa</i> (279,905 bp), in the genome sequence of <i>Penaeus</i> (<i>Litopenaeus</i>) <i>vannamei</i> breed Kehai No. 1 (ASM378908v1). This endogenous virus seemed to insert exclusively into the telomeric pentanucleotide microsatellite (TAACC/GGTTA)<sub>n</sub>. It encoded 117 putative genes, with some containing introns, such as <i>g012</i> (inhibitor of apoptosis, IAP), <i>g046</i> (crustacean hyperglycemic hormone, CHH), <i>g155</i> (innexin), <i>g158</i> (Bax inhibitor 1 like). More than a dozen <i>Nimav-1_LVa</i> genes are involved in the pathogen-host interactions. We hypothesized that <i>g046</i>, <i>g155</i>, <i>g158,</i> and <i>g227</i> (semaphorin 1A like) were recruited host genes for their roles in immune regulation. Sequence analysis indicated that a total of 43 WSSV genes belonged to the ancestral/core nimavirus gene set, including four genes reported in this study: wsv112 (dUTPase), wsv206, wsv226, and wsv308 (nucleocapsid protein). The availability of the <i>Nimav-1_LVa</i> sequence would help understand the genetic diversity, epidemiology, evolution, and virulence of WSSV.https://www.mdpi.com/2073-4425/11/1/94wssvwhite spot syndrome virus<i>nimaviridae</i><i>nimav-1_lva</i><i>dnav-1_lva</i><i>penaeus</i> (<i>litopenaeus</i>) <i>vannamei</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Weidong Bao Kathy F. J. Tang Acacia Alcivar-Warren |
spellingShingle |
Weidong Bao Kathy F. J. Tang Acacia Alcivar-Warren The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i> Genes wssv white spot syndrome virus <i>nimaviridae</i> <i>nimav-1_lva</i> <i>dnav-1_lva</i> <i>penaeus</i> (<i>litopenaeus</i>) <i>vannamei</i> |
author_facet |
Weidong Bao Kathy F. J. Tang Acacia Alcivar-Warren |
author_sort |
Weidong Bao |
title |
The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i> |
title_short |
The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i> |
title_full |
The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i> |
title_fullStr |
The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i> |
title_full_unstemmed |
The Complete Genome of an Endogenous Nimavirus (<i>Nimav-1_LVa</i>) From the Pacific Whiteleg Shrimp <i>Penaeus</i> (<i>Litopenaeus</i>) <i>Vannamei</i> |
title_sort |
complete genome of an endogenous nimavirus (<i>nimav-1_lva</i>) from the pacific whiteleg shrimp <i>penaeus</i> (<i>litopenaeus</i>) <i>vannamei</i> |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-01-01 |
description |
White spot syndrome virus (WSSV), the lone virus of the genus <i>Whispovirus</i> under the family <i>Nimaviridae</i>, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limited, partly due to its large genome and the lack of other closely related free-living viruses for comparative studies. In this study, we reconstructed a full-length endogenous nimavirus consensus genome, <i>Nimav-1_LVa</i> (279,905 bp), in the genome sequence of <i>Penaeus</i> (<i>Litopenaeus</i>) <i>vannamei</i> breed Kehai No. 1 (ASM378908v1). This endogenous virus seemed to insert exclusively into the telomeric pentanucleotide microsatellite (TAACC/GGTTA)<sub>n</sub>. It encoded 117 putative genes, with some containing introns, such as <i>g012</i> (inhibitor of apoptosis, IAP), <i>g046</i> (crustacean hyperglycemic hormone, CHH), <i>g155</i> (innexin), <i>g158</i> (Bax inhibitor 1 like). More than a dozen <i>Nimav-1_LVa</i> genes are involved in the pathogen-host interactions. We hypothesized that <i>g046</i>, <i>g155</i>, <i>g158,</i> and <i>g227</i> (semaphorin 1A like) were recruited host genes for their roles in immune regulation. Sequence analysis indicated that a total of 43 WSSV genes belonged to the ancestral/core nimavirus gene set, including four genes reported in this study: wsv112 (dUTPase), wsv206, wsv226, and wsv308 (nucleocapsid protein). The availability of the <i>Nimav-1_LVa</i> sequence would help understand the genetic diversity, epidemiology, evolution, and virulence of WSSV. |
topic |
wssv white spot syndrome virus <i>nimaviridae</i> <i>nimav-1_lva</i> <i>dnav-1_lva</i> <i>penaeus</i> (<i>litopenaeus</i>) <i>vannamei</i> |
url |
https://www.mdpi.com/2073-4425/11/1/94 |
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