Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods
A simple and reliable method of ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was developed to control the quality of <em>Radix Isatidis</em> (dried root of <em>Isatis indigotica</em>) for chemical fingerprin...
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doaj-bd19d5a0d31f4463b74f2f84e18b7ace2020-11-24T23:58:14ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-07-011379035905010.3390/ijms13079035Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric MethodsZheng-Tao WangYi-Ming LiShan-Jun HuangZhi-Yong XieRui WangYan-Hong ShiA simple and reliable method of ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was developed to control the quality of <em>Radix Isatidis</em> (dried root of <em>Isatis indigotica</em>) for chemical fingerprint analysis and quantitative analysis of eight bioactive constituents, including <em>R</em>,<em>S</em>-goitrin, progoitrin, epiprogoitrin, gluconapin, adenosine, uridine, guanosine, and hypoxanthine. In quantitative analysis, the eight components showed good regression (<em>R</em><em> </em>> 0.9997) within test ranges, and the recovery method ranged from 99.5% to 103.0%. The UPLC fingerprints of the <em>Radix Isatidis </em>samples were compared by performing chemometric procedures, including similarity analysis, hierarchical clustering analysis, and principal component analysis. The chemometric procedures classified <em>Radix Isatidis</em> and its finished products such that all samples could be successfully grouped according to crude herbs, prepared slices, and adulterant <em>Baphicacanthis cusiae</em> Rhizoma et Radix. The combination of quantitative and chromatographic fingerprint analysis can be used for the quality assessment of <em>Radix Isatidis</em><em> </em>and its finished products.http://www.mdpi.com/1422-0067/13/7/9035<em>Radix Isatidis</em><em> </em>UPLC-PDAquality controlquantitativefingerprint |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zheng-Tao Wang Yi-Ming Li Shan-Jun Huang Zhi-Yong Xie Rui Wang Yan-Hong Shi |
spellingShingle |
Zheng-Tao Wang Yi-Ming Li Shan-Jun Huang Zhi-Yong Xie Rui Wang Yan-Hong Shi Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods International Journal of Molecular Sciences <em>Radix Isatidis</em> <em> </em>UPLC-PDA quality control quantitative fingerprint |
author_facet |
Zheng-Tao Wang Yi-Ming Li Shan-Jun Huang Zhi-Yong Xie Rui Wang Yan-Hong Shi |
author_sort |
Zheng-Tao Wang |
title |
Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods |
title_short |
Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods |
title_full |
Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods |
title_fullStr |
Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods |
title_full_unstemmed |
Quantitative and Chemical Fingerprint Analysis for the Quality Evaluation of <em>Isatis indigotica</em> based on Ultra-Performance Liquid Chromatography with Photodiode Array Detector Combined with Chemometric Methods |
title_sort |
quantitative and chemical fingerprint analysis for the quality evaluation of <em>isatis indigotica</em> based on ultra-performance liquid chromatography with photodiode array detector combined with chemometric methods |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2012-07-01 |
description |
A simple and reliable method of ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was developed to control the quality of <em>Radix Isatidis</em> (dried root of <em>Isatis indigotica</em>) for chemical fingerprint analysis and quantitative analysis of eight bioactive constituents, including <em>R</em>,<em>S</em>-goitrin, progoitrin, epiprogoitrin, gluconapin, adenosine, uridine, guanosine, and hypoxanthine. In quantitative analysis, the eight components showed good regression (<em>R</em><em> </em>> 0.9997) within test ranges, and the recovery method ranged from 99.5% to 103.0%. The UPLC fingerprints of the <em>Radix Isatidis </em>samples were compared by performing chemometric procedures, including similarity analysis, hierarchical clustering analysis, and principal component analysis. The chemometric procedures classified <em>Radix Isatidis</em> and its finished products such that all samples could be successfully grouped according to crude herbs, prepared slices, and adulterant <em>Baphicacanthis cusiae</em> Rhizoma et Radix. The combination of quantitative and chromatographic fingerprint analysis can be used for the quality assessment of <em>Radix Isatidis</em><em> </em>and its finished products. |
topic |
<em>Radix Isatidis</em> <em> </em>UPLC-PDA quality control quantitative fingerprint |
url |
http://www.mdpi.com/1422-0067/13/7/9035 |
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