Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development
Summary: To study contributions of N-methyl-D-aspartate receptors (NMDARs) in presynaptic and postsynaptic neurons of the developing visual system, we microinject antisense Morpholino oligonucleotide (MO) against GluN1 into one cell of two-cell-stage Xenopus laevis embryos. The resulting bilateral s...
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doaj-bd51f93a3fe444898235283958cd98df2020-11-25T03:49:27ZengElsevierCell Reports2211-12472020-07-01324107955Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit DevelopmentPhilip Kesner0Anne Schohl1Elodie C. Warren2Fan Ma3Edward S. Ruthazer4Department of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, McGill University, Montreal QC H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, McGill University, Montreal QC H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, McGill University, Montreal QC H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, McGill University, Montreal QC H3A 2B4, CanadaDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, McGill University, Montreal QC H3A 2B4, Canada; Corresponding authorSummary: To study contributions of N-methyl-D-aspartate receptors (NMDARs) in presynaptic and postsynaptic neurons of the developing visual system, we microinject antisense Morpholino oligonucleotide (MO) against GluN1 into one cell of two-cell-stage Xenopus laevis embryos. The resulting bilateral segregation of MO induces postsynaptic NMDAR (postNMDAR) knockdown in tectal neurons on one side and presynaptic NMDAR (preNMDAR) knockdown in ganglion cells projecting to the other side. PostNMDAR knockdown reduces evoked NMDAR- and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated retinotectal currents. Although the frequency of spontaneous synaptic events is increased, the probability of evoked release is reduced. PreNMDAR knockdown results in larger evoked and unitary synaptic responses. Structurally, postNMDAR and preNMDAR knockdown produce complementary effects. Axonal arbor complexity is reduced by preNMDAR-MO and increased by postNMDAR-MO, whereas tectal dendritic arbors exhibit the inverse. The current study illustrates distinct roles for pre- and postNMDARs in circuit development and reveals extensive transsynaptic regulation of form and function.http://www.sciencedirect.com/science/article/pii/S2211124720309360Xenopusvisual systemwhole-cell electrophysiologyreceptive fieldtwo-photon microscopysynapse |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Philip Kesner Anne Schohl Elodie C. Warren Fan Ma Edward S. Ruthazer |
spellingShingle |
Philip Kesner Anne Schohl Elodie C. Warren Fan Ma Edward S. Ruthazer Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development Cell Reports Xenopus visual system whole-cell electrophysiology receptive field two-photon microscopy synapse |
author_facet |
Philip Kesner Anne Schohl Elodie C. Warren Fan Ma Edward S. Ruthazer |
author_sort |
Philip Kesner |
title |
Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development |
title_short |
Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development |
title_full |
Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development |
title_fullStr |
Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development |
title_full_unstemmed |
Postsynaptic and Presynaptic NMDARs Have Distinct Roles in Visual Circuit Development |
title_sort |
postsynaptic and presynaptic nmdars have distinct roles in visual circuit development |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2020-07-01 |
description |
Summary: To study contributions of N-methyl-D-aspartate receptors (NMDARs) in presynaptic and postsynaptic neurons of the developing visual system, we microinject antisense Morpholino oligonucleotide (MO) against GluN1 into one cell of two-cell-stage Xenopus laevis embryos. The resulting bilateral segregation of MO induces postsynaptic NMDAR (postNMDAR) knockdown in tectal neurons on one side and presynaptic NMDAR (preNMDAR) knockdown in ganglion cells projecting to the other side. PostNMDAR knockdown reduces evoked NMDAR- and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR)-mediated retinotectal currents. Although the frequency of spontaneous synaptic events is increased, the probability of evoked release is reduced. PreNMDAR knockdown results in larger evoked and unitary synaptic responses. Structurally, postNMDAR and preNMDAR knockdown produce complementary effects. Axonal arbor complexity is reduced by preNMDAR-MO and increased by postNMDAR-MO, whereas tectal dendritic arbors exhibit the inverse. The current study illustrates distinct roles for pre- and postNMDARs in circuit development and reveals extensive transsynaptic regulation of form and function. |
topic |
Xenopus visual system whole-cell electrophysiology receptive field two-photon microscopy synapse |
url |
http://www.sciencedirect.com/science/article/pii/S2211124720309360 |
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