Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen
The inducible innate immune response to infection requires a concerted process of gene expression that is regulated at multiple levels. Most global analyses of the innate immune response have focused on transcription induced by defined immunostimulatory ligands, such as lipopolysaccharide. However,...
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doaj-bd52dfd392d9417596c076236d4d05ba2021-05-05T13:23:33ZengeLife Sciences Publications LtdeLife2050-084X2017-04-01610.7554/eLife.22707Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogenKevin C Barry0https://orcid.org/0000-0003-1064-5964Nicholas T Ingolia1https://orcid.org/0000-0002-3395-1545Russell E Vance2https://orcid.org/0000-0002-6686-3912Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDivision of Biochemistry, Biophysics and Structural Biology, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDivision of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States; Cancer Research Laboratory, University of California, Berkeley, Berkeley, United States; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United StatesThe inducible innate immune response to infection requires a concerted process of gene expression that is regulated at multiple levels. Most global analyses of the innate immune response have focused on transcription induced by defined immunostimulatory ligands, such as lipopolysaccharide. However, the response to pathogens involves additional complexity, as pathogens interfere with virtually every step of gene expression. How cells respond to pathogen-mediated disruption of gene expression to nevertheless initiate protective responses remains unclear. We previously discovered that a pathogen-mediated blockade of host protein synthesis provokes the production of specific pro-inflammatory cytokines. It remains unclear how these cytokines are produced despite the global pathogen-induced block of translation. We addressed this question by using parallel RNAseq and ribosome profiling to characterize the response of macrophages to infection with the intracellular bacterial pathogen Legionella pneumophila. Our results reveal that mRNA superinduction is required for the inducible immune response to a bacterial pathogen.https://elifesciences.org/articles/22707Legionella pneumophilatranslationribosome profilingmacrophagesinnate immunity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kevin C Barry Nicholas T Ingolia Russell E Vance |
spellingShingle |
Kevin C Barry Nicholas T Ingolia Russell E Vance Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen eLife Legionella pneumophila translation ribosome profiling macrophages innate immunity |
author_facet |
Kevin C Barry Nicholas T Ingolia Russell E Vance |
author_sort |
Kevin C Barry |
title |
Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen |
title_short |
Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen |
title_full |
Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen |
title_fullStr |
Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen |
title_full_unstemmed |
Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen |
title_sort |
global analysis of gene expression reveals mrna superinduction is required for the inducible immune response to a bacterial pathogen |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2017-04-01 |
description |
The inducible innate immune response to infection requires a concerted process of gene expression that is regulated at multiple levels. Most global analyses of the innate immune response have focused on transcription induced by defined immunostimulatory ligands, such as lipopolysaccharide. However, the response to pathogens involves additional complexity, as pathogens interfere with virtually every step of gene expression. How cells respond to pathogen-mediated disruption of gene expression to nevertheless initiate protective responses remains unclear. We previously discovered that a pathogen-mediated blockade of host protein synthesis provokes the production of specific pro-inflammatory cytokines. It remains unclear how these cytokines are produced despite the global pathogen-induced block of translation. We addressed this question by using parallel RNAseq and ribosome profiling to characterize the response of macrophages to infection with the intracellular bacterial pathogen Legionella pneumophila. Our results reveal that mRNA superinduction is required for the inducible immune response to a bacterial pathogen. |
topic |
Legionella pneumophila translation ribosome profiling macrophages innate immunity |
url |
https://elifesciences.org/articles/22707 |
work_keys_str_mv |
AT kevincbarry globalanalysisofgeneexpressionrevealsmrnasuperinductionisrequiredfortheinducibleimmuneresponsetoabacterialpathogen AT nicholastingolia globalanalysisofgeneexpressionrevealsmrnasuperinductionisrequiredfortheinducibleimmuneresponsetoabacterialpathogen AT russellevance globalanalysisofgeneexpressionrevealsmrnasuperinductionisrequiredfortheinducibleimmuneresponsetoabacterialpathogen |
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