Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells
Summary: We report that cerebrum-specific inactivation of the histone deacetylase 3 (HDAC3) gene causes striking developmental defects in the neocortex, hippocampus, and corpus callosum; post-weaning lethality; and abnormal behaviors, including hyperactivity and anxiety. The defects are due to rapid...
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doaj-bd58db4b104347f0b7002657beb420872020-11-24T21:47:22ZengElsevieriScience2589-00422019-10-0120148167Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor CellsLin Li0Jianliang Jin1Xiang-Jiao Yang2The Rosalind & Morris Goodman Cancer Research Center, Montreal, QC H3A 1A3, Canada; Department of Medicine and McGill University, Montreal, QC H3A 1A3, CanadaThe Rosalind & Morris Goodman Cancer Research Center, Montreal, QC H3A 1A3, Canada; Research Center for Bone and Stem Cells, Department of Human Anatomy, Key Laboratory of Aging & Disease, Nanjing Medical University, Nanjing, Jiangsu 211166, ChinaThe Rosalind & Morris Goodman Cancer Research Center, Montreal, QC H3A 1A3, Canada; Department of Medicine and McGill University, Montreal, QC H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC H3A 1A3, Canada; Department of Medicine, McGill University Health Center, Montreal, QC H3A 1A3, Canada; Corresponding authorSummary: We report that cerebrum-specific inactivation of the histone deacetylase 3 (HDAC3) gene causes striking developmental defects in the neocortex, hippocampus, and corpus callosum; post-weaning lethality; and abnormal behaviors, including hyperactivity and anxiety. The defects are due to rapid loss of embryonic neural stem and progenitor cells (NSPCs). Premature neurogenesis and abnormal neuronal migration in the mutant brain alter NSPC homeostasis. Mutant cerebral cortices also display augmented DNA damage responses, apoptosis, and histone hyperacetylation. Moreover, mutant NSPCs are impaired in forming neurospheres in vitro, and treatment with the HDAC3-specific inhibitor RGFP966 abolishes neurosphere formation. Transcriptomic analyses of neonatal cerebral cortices and cultured neurospheres support that HDAC3 regulates transcriptional programs through interaction with different transcription factors, including NFIB. These findings establish HDAC3 as a major deacetylase critical for perinatal development of the mouse cerebrum and NSPCs, thereby suggesting a direct link of this enzymatic epigenetic regulator to human cerebral and intellectual development. : Cellular Neuroscience; Developmental Neuroscience; Molecular Neuroscience; Neuroscience Subject Areas: Cellular Neuroscience, Developmental Neuroscience, Molecular Neuroscience, Neurosciencehttp://www.sciencedirect.com/science/article/pii/S2589004219303530 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lin Li Jianliang Jin Xiang-Jiao Yang |
spellingShingle |
Lin Li Jianliang Jin Xiang-Jiao Yang Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells iScience |
author_facet |
Lin Li Jianliang Jin Xiang-Jiao Yang |
author_sort |
Lin Li |
title |
Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells |
title_short |
Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells |
title_full |
Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells |
title_fullStr |
Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells |
title_full_unstemmed |
Histone Deacetylase 3 Governs Perinatal Cerebral Development via Neural Stem and Progenitor Cells |
title_sort |
histone deacetylase 3 governs perinatal cerebral development via neural stem and progenitor cells |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2019-10-01 |
description |
Summary: We report that cerebrum-specific inactivation of the histone deacetylase 3 (HDAC3) gene causes striking developmental defects in the neocortex, hippocampus, and corpus callosum; post-weaning lethality; and abnormal behaviors, including hyperactivity and anxiety. The defects are due to rapid loss of embryonic neural stem and progenitor cells (NSPCs). Premature neurogenesis and abnormal neuronal migration in the mutant brain alter NSPC homeostasis. Mutant cerebral cortices also display augmented DNA damage responses, apoptosis, and histone hyperacetylation. Moreover, mutant NSPCs are impaired in forming neurospheres in vitro, and treatment with the HDAC3-specific inhibitor RGFP966 abolishes neurosphere formation. Transcriptomic analyses of neonatal cerebral cortices and cultured neurospheres support that HDAC3 regulates transcriptional programs through interaction with different transcription factors, including NFIB. These findings establish HDAC3 as a major deacetylase critical for perinatal development of the mouse cerebrum and NSPCs, thereby suggesting a direct link of this enzymatic epigenetic regulator to human cerebral and intellectual development. : Cellular Neuroscience; Developmental Neuroscience; Molecular Neuroscience; Neuroscience Subject Areas: Cellular Neuroscience, Developmental Neuroscience, Molecular Neuroscience, Neuroscience |
url |
http://www.sciencedirect.com/science/article/pii/S2589004219303530 |
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