An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i>
<b>Aim:</b> To evaluate and compare the antibacterial effects of chlorhexidine and calcium hydroxide on <i>Enterococcus faecalis</i>. <b>Materials and Methods:</b> Root canal treatment involves a number of steps. In spite of all the steps done thoroughly, root ca...
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doaj-bd6690afdf6042019350ae7e8fae8dfb2020-11-24T23:29:30ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032010-01-01214512514An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i>Jhamb SwatyNikhil VineetaSingh Vijay<b>Aim:</b> To evaluate and compare the antibacterial effects of chlorhexidine and calcium hydroxide on <i>Enterococcus faecalis</i>. <b>Materials and Methods:</b> Root canal treatment involves a number of steps. In spite of all the steps done thoroughly, root canal treatment might fail due to the remnant microbes. Of all such bacteria, <i>E. faecalis</i> is found in failed root canals. The study tests the antibacterial activity of various intracanal medicaments. Agar diffusion test was used to evaluate the antibacterial effects of the following antibacterial agents: i. hexidine:0.2% chlorhexidine gluconate; ii. periogard:0.12% chlorhexidine gluconate; iii. calcium hydroxide powder plus sterile water; iv. metapaste plus sterile water; v. calcium hydroxide plus hexidine; vi. calcium hydroxide plus periogard; vii. metapaste plus hexidine; viii. metapaste plus periogard. The size of zones of inhibition was measured. <b>Results:</b> The average size of zones of inhibition after 72 hours were hexidine: 5 mm; periogard: 4.25 mm; calcium hydroxide plus sterile water: 0.5 mm; metapaste plus sterile water: 0.5 mm; calcium hydroxide plus hexidine: 4.7 mm; calcium hydroxide plus periogard: 4 mm; metapaste plus hexidine: 4.65 mm; metapaste plus periogard: 4 mm. Results were subjected to statistical analysis using one way analysis of variance and Tukey tests. <b>Conclusion:</b> Chlorhexidine and its preparations are more potent antibacterial agents against<i>E. faecalis</i> in comparison to calcium hydroxide.http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=4;spage=512;epage=514;aulast=JhambAgar diffusion testchlorhexidinemetapasteperiogardtrypticase soy agar |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jhamb Swaty Nikhil Vineeta Singh Vijay |
spellingShingle |
Jhamb Swaty Nikhil Vineeta Singh Vijay An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i> Indian Journal of Dental Research Agar diffusion test chlorhexidine metapaste periogard trypticase soy agar |
author_facet |
Jhamb Swaty Nikhil Vineeta Singh Vijay |
author_sort |
Jhamb Swaty |
title |
An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i> |
title_short |
An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i> |
title_full |
An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i> |
title_fullStr |
An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i> |
title_full_unstemmed |
An <i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>Enterococcus faecalis</i> |
title_sort |
<i>in vitro</i> study of antibacterial effect of calcium hydroxide and chlorhexidine on <i>enterococcus faecalis</i> |
publisher |
Wolters Kluwer Medknow Publications |
series |
Indian Journal of Dental Research |
issn |
0970-9290 1998-3603 |
publishDate |
2010-01-01 |
description |
<b>Aim:</b> To evaluate and compare the antibacterial effects of chlorhexidine and calcium hydroxide on <i>Enterococcus faecalis</i>. <b>Materials and Methods:</b> Root canal treatment involves a number of steps. In spite of all the steps done thoroughly, root canal treatment might fail due to the remnant microbes. Of all such bacteria, <i>E. faecalis</i> is found in failed root canals. The study tests the antibacterial activity of various intracanal medicaments. Agar diffusion test was used to evaluate the antibacterial effects of the following antibacterial agents: i. hexidine:0.2% chlorhexidine gluconate; ii. periogard:0.12% chlorhexidine gluconate; iii. calcium hydroxide powder plus sterile water; iv. metapaste plus sterile water; v. calcium hydroxide plus hexidine; vi. calcium hydroxide plus periogard; vii. metapaste plus hexidine; viii. metapaste plus periogard. The size of zones of inhibition was measured. <b>Results:</b> The average size of zones of inhibition after 72 hours were hexidine: 5 mm; periogard: 4.25 mm; calcium hydroxide plus sterile water: 0.5 mm; metapaste plus sterile water: 0.5 mm; calcium hydroxide plus hexidine: 4.7 mm; calcium hydroxide plus periogard: 4 mm; metapaste plus hexidine: 4.65 mm; metapaste plus periogard: 4 mm. Results were subjected to statistical analysis using one way analysis of variance and Tukey tests. <b>Conclusion:</b> Chlorhexidine and its preparations are more potent antibacterial agents against<i>E. faecalis</i> in comparison to calcium hydroxide. |
topic |
Agar diffusion test chlorhexidine metapaste periogard trypticase soy agar |
url |
http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=4;spage=512;epage=514;aulast=Jhamb |
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