Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification

Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification

The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on in...

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Bibliographic Details
Main Authors: Ming Xu, Jiping Li, Ying Dong, Ning Liu, Jiayu Wan, Wensen Liu, Yucheng Sun, Hongwei Gao, Linna Liu
Format: Article
Language:English
Published: MDPI AG 2009-05-01
Series:International Journal of Molecular Sciences
Subjects:
ricin
BBM
SPR
HPCE-ESI-MS
Online Access:http://www.mdpi.com/1422-0067/10/5/2431/
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spelling doaj-bdb92058c0cb40448af4b5d0eb75a9d52020-11-24T23:56:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672009-05-011052431243910.3390/ijms10052431Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS IdentificationMing XuJiping LiYing DongNing LiuJiayu WanWensen LiuYucheng SunHongwei GaoLinna LiuThe present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin. http://www.mdpi.com/1422-0067/10/5/2431/ricinBBMSPRHPCE-ESI-MS
collection DOAJ
language English
format Article
sources DOAJ
author Ming Xu
Jiping Li
Ying Dong
Ning Liu
Jiayu Wan
Wensen Liu
Yucheng Sun
Hongwei Gao
Linna Liu
spellingShingle Ming Xu
Jiping Li
Ying Dong
Ning Liu
Jiayu Wan
Wensen Liu
Yucheng Sun
Hongwei Gao
Linna Liu
Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
International Journal of Molecular Sciences
ricin
BBM
SPR
HPCE-ESI-MS
author_facet Ming Xu
Jiping Li
Ying Dong
Ning Liu
Jiayu Wan
Wensen Liu
Yucheng Sun
Hongwei Gao
Linna Liu
author_sort Ming Xu
title Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_short Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_full Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_fullStr Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_full_unstemmed Analysis of Intestinal Injuries Induced by Ricin in Vitro Using SPR Technology and MS Identification
title_sort analysis of intestinal injuries induced by ricin in vitro using spr technology and ms identification
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2009-05-01
description The present study found that ricin toxicity did not only manifest itself as inhibition of protein synthesis, but also induced apoptosis of immune cells and played an extremely significant role in intestinal injury. In this report, we describe a novel method to estimate binding events occurring on intestinal brush border membranes (BBM) based on SPR technology in an attempt to mimic the real intestinal surface capable of interacting physically and/or actively with certain biological molecules. Combined with HPCE-ESI-MS indentification, we obtained 28 kinds of proteins in BBM that interacted with ricin.
topic ricin
BBM
SPR
HPCE-ESI-MS
url http://www.mdpi.com/1422-0067/10/5/2431/
work_keys_str_mv AT mingxu analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT jipingli analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT yingdong analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT ningliu analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT jiayuwan analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT wensenliu analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT yuchengsun analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT hongweigao analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
AT linnaliu analysisofintestinalinjuriesinducedbyricininvitrousingsprtechnologyandmsidentification
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