The Pro12Ala polymorphism in the PPAR­γ2 gene is not associated with an increased risk of NAFLD in Iranian patients with type 2 diabetes mellitus

• Main Authors: Leila Saremi, Shirin Lotfipanah, Masumeh Mohammadi, Hassan Hosseinzadeh, Mina Fathi-Kazerooni, Behrooz Johari, Zohreh Saltanatpour
• Format: Article
• Language: English
• Published: BMC 2019-03-01
• Series: Cellular & Molecular Biology Letters
• Subjects: Pro12Ala polymorphism; PPAR­γ2 gene; NAFLD; Type 2 diabetes mellitus
• Online Access: http://link.springer.com/article/10.1186/s11658-019-0138-0
• ISSN: 1425-8153; 1689-1392

Abstract Background The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. Several studies have demonstrated a significant association between Pro12Ala polymorphism of the PPAR­γ2 gene and metabolic disorders. Therefore, this study aimed to evaluate the association of Pro12Ala polymorphism with increased risk of NAFLD in Iranian patients with type 2 diabetes mellitus. Methods This cross-sectional study was performed on 145 healthy control subjects and 145 NAFLD patients with a history of type 2 diabetes. Pro12Ala polymorphism genotyping was performed using PCR–restriction fragment length polymorphism (RFLP) technique with the Bs1I restriction enzyme. Results Our results demonstrated that CC and GG genotypes of Pro12Ala were found in the participants, but there was no statistically significant difference between NAFLD patients and healthy controls (P = 0.64 and χ2 = 0.21). Conclusion This study suggests that Pro12Ala polymorphism of the PPAR­γ2 gene cannot be considered as a risk factor for NAFLD in the Iranian population.