Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway
Signaling through the mammalian target of rapamycin (mTOR) in response to leucine modulates many cellular and developmental processes. However, in the context of satellite cell proliferation and differentiation, the role of leucine and mTORC1 is less known. This study investigates the role of leucin...
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doaj-bdc0e95d4ce0439f936de7a00fdf98692020-11-25T02:30:51ZengMDPI AGNutrients2072-66432015-05-01753387340010.3390/nu7053387nu7053387Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling PathwayJie-Min Dai0Mu-Xue Yu1Zhen-Yu Shen2Chu-Yi Guo3Si-Qi Zhuang4Xiao-Shan Qiu5Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, ChinaDepartment of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province 510080, ChinaSignaling through the mammalian target of rapamycin (mTOR) in response to leucine modulates many cellular and developmental processes. However, in the context of satellite cell proliferation and differentiation, the role of leucine and mTORC1 is less known. This study investigates the role of leucine in the process of proliferation and differentiation of primary preterm rat satellite cells, and the relationship with mammalian target of rapamycin complex 1 (mTORC1) activation. Dissociation of primary satellite cells occurred with type I collagenase and trypsin, and purification, via different speed adherence methods. Satellite cells with positive expression of Desmin were treated with leucine and rapamycin. We observed that leucine promoted proliferation and differentiation of primary satellite cells and increased the phosphorylation of mTOR. Rapamycin inhibited proliferation and differentiation, as well as decreased the phosphorylation level of mTOR. Furthermore, leucine increased the expression of MyoD and myogenin while the protein level of MyoD decreased due to rapamycin. However, myogenin expressed no affect by rapamycin. In conclusion, leucine may up-regulate the activation of mTORC1 to promote proliferation and differentiation of primary preterm rat satellite cells. We have shown that leucine promoted the differentiation of myotubes in part through the mTORC1-MyoD signal pathway.http://www.mdpi.com/2072-6643/7/5/3387leucinepretermsatellite cellsproliferationdifferentiationmTORMyoDmyogenin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jie-Min Dai Mu-Xue Yu Zhen-Yu Shen Chu-Yi Guo Si-Qi Zhuang Xiao-Shan Qiu |
spellingShingle |
Jie-Min Dai Mu-Xue Yu Zhen-Yu Shen Chu-Yi Guo Si-Qi Zhuang Xiao-Shan Qiu Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway Nutrients leucine preterm satellite cells proliferation differentiation mTOR MyoD myogenin |
author_facet |
Jie-Min Dai Mu-Xue Yu Zhen-Yu Shen Chu-Yi Guo Si-Qi Zhuang Xiao-Shan Qiu |
author_sort |
Jie-Min Dai |
title |
Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway |
title_short |
Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway |
title_full |
Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway |
title_fullStr |
Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway |
title_full_unstemmed |
Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway |
title_sort |
leucine promotes proliferation and differentiation of primary preterm rat satellite cells in part through mtorc1 signaling pathway |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2015-05-01 |
description |
Signaling through the mammalian target of rapamycin (mTOR) in response to leucine modulates many cellular and developmental processes. However, in the context of satellite cell proliferation and differentiation, the role of leucine and mTORC1 is less known. This study investigates the role of leucine in the process of proliferation and differentiation of primary preterm rat satellite cells, and the relationship with mammalian target of rapamycin complex 1 (mTORC1) activation. Dissociation of primary satellite cells occurred with type I collagenase and trypsin, and purification, via different speed adherence methods. Satellite cells with positive expression of Desmin were treated with leucine and rapamycin. We observed that leucine promoted proliferation and differentiation of primary satellite cells and increased the phosphorylation of mTOR. Rapamycin inhibited proliferation and differentiation, as well as decreased the phosphorylation level of mTOR. Furthermore, leucine increased the expression of MyoD and myogenin while the protein level of MyoD decreased due to rapamycin. However, myogenin expressed no affect by rapamycin. In conclusion, leucine may up-regulate the activation of mTORC1 to promote proliferation and differentiation of primary preterm rat satellite cells. We have shown that leucine promoted the differentiation of myotubes in part through the mTORC1-MyoD signal pathway. |
topic |
leucine preterm satellite cells proliferation differentiation mTOR MyoD myogenin |
url |
http://www.mdpi.com/2072-6643/7/5/3387 |
work_keys_str_mv |
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