Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR
Streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. The aim of this study was to evaluate PCR assay compared with conventional culture method for direct detection of Streptococcus agalactiae. Total of 203 paired low vaginal swabs were collected from wome...
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Tehran University of Medical Sciences
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doaj-bdf1aacc71db48ba851b17af2dc2cad92020-11-25T03:26:02ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942017-01-0154125091Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCRSeyed Masoud Mousavi0Seyed Mostafa Hosseini1Rasoul Yousefi Mashouf2Mohammad Reza Arabestani3Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. AND Department of Microbiology, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. The aim of this study was to evaluate PCR assay compared with conventional culture method for direct detection of Streptococcus agalactiae. Total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from June 2013 through February 2014 for detection of Streptococcus agalactiae using PCR assay targeting 16S rRNA, cfb, scpB, and atr genes and culture method following broth enrichment. The results were recorded and evaluated for determining of sensitivity, specificity, positive and negative predictive values of PCR assaycompared with culture method. Prevalence of Streptococcus agalactiae was determined as 7.39% (n=15) using culture method; 19.70% (n=40) by PCR targeting 16S rRNA gene; 18.23% (n=37) by targeting atr gene; 17.24% (n=35) by cfb gene; and 8.87% (n=18) by scpB gene. Generally, a total of 49 specimens were considered true positive (27 samples by PCR assay using the four genes in sum, 4 samples only by atr gene PCR, 3 samples only by cfb gene PCR, 2 samples only by culture method, and 13 samples by PCR assay and culture method in common) and prevalence of Streptococcus agalactiae determined 24.14% in Hamadan. The current data demonstrated that performing only culture method for detecting GBS from pregnant women leads to missed false negative carrier individuals. Thus, it is recommended that both the PCR assay and conventional culture method to be performed in order to detect Streptococcus agalactiae. https://acta.tums.ac.ir/index.php/acta/article/view/6105Streptococcus agalactiaePregnancyPolymerase chain reaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seyed Masoud Mousavi Seyed Mostafa Hosseini Rasoul Yousefi Mashouf Mohammad Reza Arabestani |
spellingShingle |
Seyed Masoud Mousavi Seyed Mostafa Hosseini Rasoul Yousefi Mashouf Mohammad Reza Arabestani Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR Acta Medica Iranica Streptococcus agalactiae Pregnancy Polymerase chain reaction |
author_facet |
Seyed Masoud Mousavi Seyed Mostafa Hosseini Rasoul Yousefi Mashouf Mohammad Reza Arabestani |
author_sort |
Seyed Masoud Mousavi |
title |
Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR |
title_short |
Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR |
title_full |
Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR |
title_fullStr |
Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR |
title_full_unstemmed |
Identification of Group B Streptococci Using 16S rRNA, cfb, scpB, and atr Genes in Pregnant Women by PCR |
title_sort |
identification of group b streptococci using 16s rrna, cfb, scpb, and atr genes in pregnant women by pcr |
publisher |
Tehran University of Medical Sciences |
series |
Acta Medica Iranica |
issn |
0044-6025 1735-9694 |
publishDate |
2017-01-01 |
description |
Streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. The aim of this study was to evaluate PCR assay compared with conventional culture method for direct detection of Streptococcus agalactiae. Total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from June 2013 through February 2014 for detection of Streptococcus agalactiae using PCR assay targeting 16S rRNA, cfb, scpB, and atr genes and culture method following broth enrichment. The results were recorded and evaluated for determining of sensitivity, specificity, positive and negative predictive values of PCR assaycompared with culture method. Prevalence of Streptococcus agalactiae was determined as 7.39% (n=15) using culture method; 19.70% (n=40) by PCR targeting 16S rRNA gene; 18.23% (n=37) by targeting atr gene; 17.24% (n=35) by cfb gene; and 8.87% (n=18) by scpB gene. Generally, a total of 49 specimens were considered true positive (27 samples by PCR assay using the four genes in sum, 4 samples only by atr gene PCR, 3 samples only by cfb gene PCR, 2 samples only by culture method, and 13 samples by PCR assay and culture method in common) and prevalence of Streptococcus agalactiae determined 24.14% in Hamadan. The current data demonstrated that performing only culture method for detecting GBS from pregnant women leads to missed false negative carrier individuals. Thus, it is recommended that both the PCR assay and conventional culture method to be performed in order to detect Streptococcus agalactiae.
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topic |
Streptococcus agalactiae Pregnancy Polymerase chain reaction |
url |
https://acta.tums.ac.ir/index.php/acta/article/view/6105 |
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