Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy
The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable carboxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhib...
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doaj-bdf5871752324ba38b6175bcc9837a682021-07-15T15:43:39ZengMDPI AGPolymers2073-43602021-06-01132129212910.3390/polym13132129Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after LaminectomyHsuan-Yu Chen0Tzu-Chieh Lin1Chih-Yung Chiang2Shiuan-Li Wey3Feng-Huei Lin4Kai-Chiang Yang5Chih-Hao Chang6Ming-Hsiao Hu7Department of Biomedical Engineering, National Taiwan University, Taipei 106216, TaiwanDepartment of Biomedical Engineering, National Taiwan University, Taipei 106216, TaiwanDepartment of Biomedical Engineering, National Taiwan University, Taipei 106216, TaiwanDepartment of Pathology, Hsinchu Mackay Memorial Hospital, HsinChu City 30071, TaiwanDepartment of Biomedical Engineering, National Taiwan University, Taipei 106216, TaiwanDepartment of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 11031, TaiwanDepartment of Orthopedics, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei 100225, TaiwanDepartment of Orthopedics, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei 100225, TaiwanThe application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable carboxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompatibility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expansion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy.https://www.mdpi.com/2073-4360/13/13/2129epidural fibrosislaminectomy<i>Bletilla striata</i>carboxymethyl celluloseresveratrol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hsuan-Yu Chen Tzu-Chieh Lin Chih-Yung Chiang Shiuan-Li Wey Feng-Huei Lin Kai-Chiang Yang Chih-Hao Chang Ming-Hsiao Hu |
spellingShingle |
Hsuan-Yu Chen Tzu-Chieh Lin Chih-Yung Chiang Shiuan-Li Wey Feng-Huei Lin Kai-Chiang Yang Chih-Hao Chang Ming-Hsiao Hu Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy Polymers epidural fibrosis laminectomy <i>Bletilla striata</i> carboxymethyl cellulose resveratrol |
author_facet |
Hsuan-Yu Chen Tzu-Chieh Lin Chih-Yung Chiang Shiuan-Li Wey Feng-Huei Lin Kai-Chiang Yang Chih-Hao Chang Ming-Hsiao Hu |
author_sort |
Hsuan-Yu Chen |
title |
Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy |
title_short |
Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy |
title_full |
Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy |
title_fullStr |
Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy |
title_full_unstemmed |
Antifibrotic Effect of <i>Bletilla striata</i> Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy |
title_sort |
antifibrotic effect of <i>bletilla striata</i> polysaccharide-resveratrol-impregnated dual-layer carboxymethyl cellulose-based sponge for the prevention of epidural fibrosis after laminectomy |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2021-06-01 |
description |
The application of antifibrotic materials can alleviate epidural fibrosis by restricting excessive fibroblast proliferation and mitigating scar tissue formation. Here, a biodegradable carboxymethyl cellulose (CMC)-Bletilla striata polysaccharide (BSP)-resveratrol (RES) sponge was fabricated to inhibit scar tissue formation post laminectomy surgery. Fibroblasts NIH/3T3, myoblasts C2C12, neural cells PC-12, and Schwann cells RSC96 were used to evaluate the in vitro cytocompatibility. Laminectomies on 10 Sprague–Dawley rats with/without the application of the CMC-BSP-RES sponge were performed. The severity of adhesion between the dura mater and formed scar tissue was qualitatively scored. All cell lines exhibited good viability with no significant difference in cytotoxicity when cultured with variable extractions of the CMC-BSP-RES sponge. S100a4 and P4hb expressions were downregulated in NIH/3T3 cultured in the CMC-BSP-RES sponge, implying that this sponge potentially inhibits fibroblast activity. No post-operative shrinkage or dura mater expansion along the surgical site was detected. The peel-off tests revealed that the tenacity of adhesion de-creased. Histopathological examinations verified that the average number of fibroblasts in the CMC-BSP-RES group considerably decreased. The CMC-BSP-RES sponge is a biocompatible and effective material for alleviating post-operative epidural fibrosis and mitigating fibroblast expression following laminectomy. |
topic |
epidural fibrosis laminectomy <i>Bletilla striata</i> carboxymethyl cellulose resveratrol |
url |
https://www.mdpi.com/2073-4360/13/13/2129 |
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