MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner
Summary: Dendritic cells, cells of the innate immune system, are found in a steady state poised to respond to activating stimuli. Once stimulated, they rapidly undergo dynamic changes in gene expression to adopt an activated phenotype capable of stimulating immune responses. We find that the microRN...
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2020-05-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720305349 |
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doaj-bdfb2ccc8fcf48c08209ddf4aa1116592020-11-25T02:40:46ZengElsevierCell Reports2211-12472020-05-01315MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific MannerBrendan Cordeiro0Peter Jeon1Giselle M. Boukhaled2Mario Corrado3Orsolya Lapohos4Dominic G. Roy5Kelsey Williams6Russell G. Jones7Samantha Gruenheid8Selena M. Sagan9Connie M. Krawczyk10Department of Microbiology and Immunology, McGill University, Montreal, QC H3G 1Y6, CanadaGoodman Cancer Research Center, Department of Physiology, McGill University, Montreal, QC H3G 1Y6, CanadaGoodman Cancer Research Center, Department of Physiology, McGill University, Montreal, QC H3G 1Y6, CanadaGoodman Cancer Research Center, Department of Physiology, McGill University, Montreal, QC H3G 1Y6, CanadaDepartment of Microbiology and Immunology, McGill University, Montreal, QC H3G 1Y6, CanadaGoodman Cancer Research Center, Department of Physiology, McGill University, Montreal, QC H3G 1Y6, CanadaMetabolic and Nutritional Programming Group, Van Andel Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USAGoodman Cancer Research Center, Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada; Metabolic and Nutritional Programming Group, Van Andel Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USADepartment of Microbiology and Immunology, McGill University, Montreal, QC H3G 1Y6, CanadaDepartment of Microbiology and Immunology, McGill University, Montreal, QC H3G 1Y6, CanadaDepartment of Microbiology and Immunology, McGill University, Montreal, QC H3G 1Y6, Canada; Goodman Cancer Research Center, Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada; Metabolic and Nutritional Programming Group, Van Andel Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USA; Corresponding authorSummary: Dendritic cells, cells of the innate immune system, are found in a steady state poised to respond to activating stimuli. Once stimulated, they rapidly undergo dynamic changes in gene expression to adopt an activated phenotype capable of stimulating immune responses. We find that the microRNA miR-9 is upregulated in both bone marrow-derived DCs and conventional DC1s but not in conventional DC2s following stimulation. miR-9 expression in BMDCs and conventional DC1s promotes enhanced DC activation and function, including the ability to stimulate T cell activation and control tumor growth. We find that miR-9 regulated the expression of several negative regulators of transcription, including the transcriptional repressor Polycomb group factor 6 (Pcgf6). These findings demonstrate that miR-9 facilitates the transition of DCs from steady state to mature state by regulating the expression of several negative regulators of DC function in a cell-type-specific manner.http://www.sciencedirect.com/science/article/pii/S2211124720305349dendritic cellsmicroRNAmiR-9PCGF6transcriptional regulatorstumor |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Brendan Cordeiro Peter Jeon Giselle M. Boukhaled Mario Corrado Orsolya Lapohos Dominic G. Roy Kelsey Williams Russell G. Jones Samantha Gruenheid Selena M. Sagan Connie M. Krawczyk |
| spellingShingle |
Brendan Cordeiro Peter Jeon Giselle M. Boukhaled Mario Corrado Orsolya Lapohos Dominic G. Roy Kelsey Williams Russell G. Jones Samantha Gruenheid Selena M. Sagan Connie M. Krawczyk MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner Cell Reports dendritic cells microRNA miR-9 PCGF6 transcriptional regulators tumor |
| author_facet |
Brendan Cordeiro Peter Jeon Giselle M. Boukhaled Mario Corrado Orsolya Lapohos Dominic G. Roy Kelsey Williams Russell G. Jones Samantha Gruenheid Selena M. Sagan Connie M. Krawczyk |
| author_sort |
Brendan Cordeiro |
| title |
MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner |
| title_short |
MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner |
| title_full |
MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner |
| title_fullStr |
MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner |
| title_full_unstemmed |
MicroRNA-9 Fine-Tunes Dendritic Cell Function by Suppressing Negative Regulators in a Cell-Type-Specific Manner |
| title_sort |
microrna-9 fine-tunes dendritic cell function by suppressing negative regulators in a cell-type-specific manner |
| publisher |
Elsevier |
| series |
Cell Reports |
| issn |
2211-1247 |
| publishDate |
2020-05-01 |
| description |
Summary: Dendritic cells, cells of the innate immune system, are found in a steady state poised to respond to activating stimuli. Once stimulated, they rapidly undergo dynamic changes in gene expression to adopt an activated phenotype capable of stimulating immune responses. We find that the microRNA miR-9 is upregulated in both bone marrow-derived DCs and conventional DC1s but not in conventional DC2s following stimulation. miR-9 expression in BMDCs and conventional DC1s promotes enhanced DC activation and function, including the ability to stimulate T cell activation and control tumor growth. We find that miR-9 regulated the expression of several negative regulators of transcription, including the transcriptional repressor Polycomb group factor 6 (Pcgf6). These findings demonstrate that miR-9 facilitates the transition of DCs from steady state to mature state by regulating the expression of several negative regulators of DC function in a cell-type-specific manner. |
| topic |
dendritic cells microRNA miR-9 PCGF6 transcriptional regulators tumor |
| url |
http://www.sciencedirect.com/science/article/pii/S2211124720305349 |
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