Nova técnica para contagem do número de células de blastocistos
A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture,...
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Universidade Federal de Minas Gerais
2010-12-01
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doaj-bdfce9ac66224cbab54544b6a1d59fa62020-11-25T00:26:14ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622010-12-016261507151010.1590/S0102-09352010000600032S0102-09352010000600032Nova técnica para contagem do número de células de blastocistosE.P. Costa0F.G. Lopes1E.C.M. Pereira2V.L.D. Queiroz3G.G. Macedo4J.R.M. Almeida Neto5A.H.A. Costa6Universidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaUniversidade Federal de ViçosaGERMOVETA simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of counting. The process was done after embryo fixation in a sodium citrate solution, and adherence in glass slide. Following, the coloration was done using a fast panoptic coloration kit. As a result, it was possible to identify the blastomeres and count them in each blastocyst. This method provided a fast and effective analysis of the total cell number when compared with other techniques. Moreover, this new method shows advantages related to the cell visualization, which can be done in more simple equipment like stereoscopic microscope. Other interesting observed point was the long period of time and quality that the coloration stays on slides, considering other techniques.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600032&lng=en&tlng=encontagem de célulasembriãoreprodução |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
E.P. Costa F.G. Lopes E.C.M. Pereira V.L.D. Queiroz G.G. Macedo J.R.M. Almeida Neto A.H.A. Costa |
spellingShingle |
E.P. Costa F.G. Lopes E.C.M. Pereira V.L.D. Queiroz G.G. Macedo J.R.M. Almeida Neto A.H.A. Costa Nova técnica para contagem do número de células de blastocistos Arquivo Brasileiro de Medicina Veterinária e Zootecnia contagem de células embrião reprodução |
author_facet |
E.P. Costa F.G. Lopes E.C.M. Pereira V.L.D. Queiroz G.G. Macedo J.R.M. Almeida Neto A.H.A. Costa |
author_sort |
E.P. Costa |
title |
Nova técnica para contagem do número de células de blastocistos |
title_short |
Nova técnica para contagem do número de células de blastocistos |
title_full |
Nova técnica para contagem do número de células de blastocistos |
title_fullStr |
Nova técnica para contagem do número de células de blastocistos |
title_full_unstemmed |
Nova técnica para contagem do número de células de blastocistos |
title_sort |
nova técnica para contagem do número de células de blastocistos |
publisher |
Universidade Federal de Minas Gerais |
series |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
issn |
1678-4162 |
publishDate |
2010-12-01 |
description |
A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of counting. The process was done after embryo fixation in a sodium citrate solution, and adherence in glass slide. Following, the coloration was done using a fast panoptic coloration kit. As a result, it was possible to identify the blastomeres and count them in each blastocyst. This method provided a fast and effective analysis of the total cell number when compared with other techniques. Moreover, this new method shows advantages related to the cell visualization, which can be done in more simple equipment like stereoscopic microscope. Other interesting observed point was the long period of time and quality that the coloration stays on slides, considering other techniques. |
topic |
contagem de células embrião reprodução |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600032&lng=en&tlng=en |
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