Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility
Abstract Background Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell wall...
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doaj-be1dbfb7aa124888bbfc075a2cb4d7a12020-11-25T03:36:45ZengBMCBiotechnology for Biofuels1754-68342019-05-0112111410.1186/s13068-019-1450-7Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibilityWagner Rodrigo de Souza0Thályta Fraga Pacheco1Karoline Estefani Duarte2Bruno Leite Sampaio3Patrícia Abrão de Oliveira Molinari4Polyana Kelly Martins5Thaís Ribeiro Santiago6Eduardo Fernandes Formighieri7Felipe Vinecky8Ana Paula Ribeiro9Bárbara Andrade Dias Brito da Cunha10Adilson Kenji Kobayashi11Rowan Andrew Craig Mitchell12Dasciana de Sousa Rodrigues Gambetta13Hugo Bruno Correa Molinari14Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Plant Sciences, Rothamsted ResearchGenetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE)Abstract Background Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production.http://link.springer.com/article/10.1186/s13068-019-1450-7SugarcaneCell-wall acylationBiomassLignocellulosic feedstockBiofuels |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wagner Rodrigo de Souza Thályta Fraga Pacheco Karoline Estefani Duarte Bruno Leite Sampaio Patrícia Abrão de Oliveira Molinari Polyana Kelly Martins Thaís Ribeiro Santiago Eduardo Fernandes Formighieri Felipe Vinecky Ana Paula Ribeiro Bárbara Andrade Dias Brito da Cunha Adilson Kenji Kobayashi Rowan Andrew Craig Mitchell Dasciana de Sousa Rodrigues Gambetta Hugo Bruno Correa Molinari |
spellingShingle |
Wagner Rodrigo de Souza Thályta Fraga Pacheco Karoline Estefani Duarte Bruno Leite Sampaio Patrícia Abrão de Oliveira Molinari Polyana Kelly Martins Thaís Ribeiro Santiago Eduardo Fernandes Formighieri Felipe Vinecky Ana Paula Ribeiro Bárbara Andrade Dias Brito da Cunha Adilson Kenji Kobayashi Rowan Andrew Craig Mitchell Dasciana de Sousa Rodrigues Gambetta Hugo Bruno Correa Molinari Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility Biotechnology for Biofuels Sugarcane Cell-wall acylation Biomass Lignocellulosic feedstock Biofuels |
author_facet |
Wagner Rodrigo de Souza Thályta Fraga Pacheco Karoline Estefani Duarte Bruno Leite Sampaio Patrícia Abrão de Oliveira Molinari Polyana Kelly Martins Thaís Ribeiro Santiago Eduardo Fernandes Formighieri Felipe Vinecky Ana Paula Ribeiro Bárbara Andrade Dias Brito da Cunha Adilson Kenji Kobayashi Rowan Andrew Craig Mitchell Dasciana de Sousa Rodrigues Gambetta Hugo Bruno Correa Molinari |
author_sort |
Wagner Rodrigo de Souza |
title |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility |
title_short |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility |
title_full |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility |
title_fullStr |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility |
title_full_unstemmed |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility |
title_sort |
silencing of a bahd acyltransferase in sugarcane increases biomass digestibility |
publisher |
BMC |
series |
Biotechnology for Biofuels |
issn |
1754-6834 |
publishDate |
2019-05-01 |
description |
Abstract Background Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. |
topic |
Sugarcane Cell-wall acylation Biomass Lignocellulosic feedstock Biofuels |
url |
http://link.springer.com/article/10.1186/s13068-019-1450-7 |
work_keys_str_mv |
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