Engineered ribosomes with tethered subunits for expanding biological function
Ribo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.
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Nature Publishing Group
2019-09-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-11427-y |
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doaj-be2becf5c8ec41ae9cede7fac7cd5d5c2021-05-11T11:41:33ZengNature Publishing GroupNature Communications2041-17232019-09-0110111310.1038/s41467-019-11427-yEngineered ribosomes with tethered subunits for expanding biological functionErik D. Carlson0Anne E. d’Aquino1Do Soon Kim2Emily M. Fulk3Kim Hoang4Teresa Szal5Alexander S. Mankin6Michael C. Jewett7Department of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityCenter for Pharmaceutical Biotechnology, University of Illinois at ChicagoCenter for Pharmaceutical Biotechnology, University of Illinois at ChicagoDepartment of Chemical and Biological Engineering, Northwestern UniversityRibo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.https://doi.org/10.1038/s41467-019-11427-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Erik D. Carlson Anne E. d’Aquino Do Soon Kim Emily M. Fulk Kim Hoang Teresa Szal Alexander S. Mankin Michael C. Jewett |
spellingShingle |
Erik D. Carlson Anne E. d’Aquino Do Soon Kim Emily M. Fulk Kim Hoang Teresa Szal Alexander S. Mankin Michael C. Jewett Engineered ribosomes with tethered subunits for expanding biological function Nature Communications |
author_facet |
Erik D. Carlson Anne E. d’Aquino Do Soon Kim Emily M. Fulk Kim Hoang Teresa Szal Alexander S. Mankin Michael C. Jewett |
author_sort |
Erik D. Carlson |
title |
Engineered ribosomes with tethered subunits for expanding biological function |
title_short |
Engineered ribosomes with tethered subunits for expanding biological function |
title_full |
Engineered ribosomes with tethered subunits for expanding biological function |
title_fullStr |
Engineered ribosomes with tethered subunits for expanding biological function |
title_full_unstemmed |
Engineered ribosomes with tethered subunits for expanding biological function |
title_sort |
engineered ribosomes with tethered subunits for expanding biological function |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2019-09-01 |
description |
Ribo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression. |
url |
https://doi.org/10.1038/s41467-019-11427-y |
work_keys_str_mv |
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