Engineered ribosomes with tethered subunits for expanding biological function

Ribo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.

Bibliographic Details
Main Authors:	Erik D. Carlson, Anne E. d’Aquino, Do Soon Kim, Emily M. Fulk, Kim Hoang, Teresa Szal, Alexander S. Mankin, Michael C. Jewett
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-09-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-11427-y

id	doaj-be2becf5c8ec41ae9cede7fac7cd5d5c
record_format	Article
spelling	doaj-be2becf5c8ec41ae9cede7fac7cd5d5c2021-05-11T11:41:33ZengNature Publishing GroupNature Communications2041-17232019-09-0110111310.1038/s41467-019-11427-yEngineered ribosomes with tethered subunits for expanding biological functionErik D. Carlson0Anne E. d’Aquino1Do Soon Kim2Emily M. Fulk3Kim Hoang4Teresa Szal5Alexander S. Mankin6Michael C. Jewett7Department of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Chemical and Biological Engineering, Northwestern UniversityCenter for Pharmaceutical Biotechnology, University of Illinois at ChicagoCenter for Pharmaceutical Biotechnology, University of Illinois at ChicagoDepartment of Chemical and Biological Engineering, Northwestern UniversityRibo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.https://doi.org/10.1038/s41467-019-11427-y
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Erik D. Carlson Anne E. d’Aquino Do Soon Kim Emily M. Fulk Kim Hoang Teresa Szal Alexander S. Mankin Michael C. Jewett
spellingShingle	Erik D. Carlson Anne E. d’Aquino Do Soon Kim Emily M. Fulk Kim Hoang Teresa Szal Alexander S. Mankin Michael C. Jewett Engineered ribosomes with tethered subunits for expanding biological function Nature Communications
author_facet	Erik D. Carlson Anne E. d’Aquino Do Soon Kim Emily M. Fulk Kim Hoang Teresa Szal Alexander S. Mankin Michael C. Jewett
author_sort	Erik D. Carlson
title	Engineered ribosomes with tethered subunits for expanding biological function
title_short	Engineered ribosomes with tethered subunits for expanding biological function
title_full	Engineered ribosomes with tethered subunits for expanding biological function
title_fullStr	Engineered ribosomes with tethered subunits for expanding biological function
title_full_unstemmed	Engineered ribosomes with tethered subunits for expanding biological function
title_sort	engineered ribosomes with tethered subunits for expanding biological function
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-09-01
description	Ribo-T is a tethered ribosome complex capable of orthogonal ribosome-mRNA functionality, but has low activity. Here the authors evolve new tether designs that support faster growth and increased protein expression.
url	https://doi.org/10.1038/s41467-019-11427-y
work_keys_str_mv	AT erikdcarlson engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT anneedaquino engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT dosoonkim engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT emilymfulk engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT kimhoang engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT teresaszal engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT alexandersmankin engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction AT michaelcjewett engineeredribosomeswithtetheredsubunitsforexpandingbiologicalfunction
_version_	1721446110271111168

Engineered ribosomes with tethered subunits for expanding biological function

Similar Items