How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment?
The positive roles of earthworms on soil functionality has been extensively documented. The capacity of the earthworm gut microbiota on decomposition and nutrient cycling under long-term fertilization in field conditions has rarely been studied. Here, we report the structural, taxonomic, and functio...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-11-01
|
Series: | Ecotoxicology and Environmental Safety |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321007557 |
id |
doaj-be755bc88c8e454e8c8575d9413338c4 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qing-Fang Bi Bing-Jie Jin Dong Zhu Yu-Gen Jiang Bang-Xiao Zheng Patrick O'Connor Xiao-Ru Yang Andreas Richter Xian-Yong Lin Yong-Guan Zhu |
spellingShingle |
Qing-Fang Bi Bing-Jie Jin Dong Zhu Yu-Gen Jiang Bang-Xiao Zheng Patrick O'Connor Xiao-Ru Yang Andreas Richter Xian-Yong Lin Yong-Guan Zhu How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? Ecotoxicology and Environmental Safety Organic-inorganic fertilization Earthworm species Gut microbiota Functional genes 16S rRNA |
author_facet |
Qing-Fang Bi Bing-Jie Jin Dong Zhu Yu-Gen Jiang Bang-Xiao Zheng Patrick O'Connor Xiao-Ru Yang Andreas Richter Xian-Yong Lin Yong-Guan Zhu |
author_sort |
Qing-Fang Bi |
title |
How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? |
title_short |
How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? |
title_full |
How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? |
title_fullStr |
How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? |
title_full_unstemmed |
How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? |
title_sort |
how can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment? |
publisher |
Elsevier |
series |
Ecotoxicology and Environmental Safety |
issn |
0147-6513 |
publishDate |
2021-11-01 |
description |
The positive roles of earthworms on soil functionality has been extensively documented. The capacity of the earthworm gut microbiota on decomposition and nutrient cycling under long-term fertilization in field conditions has rarely been studied. Here, we report the structural, taxonomic, and functional responses of Eisenia foetida and Pheretima guillelmi gut microbiota to different fertilization regimes and durations using 16S rRNA gene-based Illumina sequencing and high-throughput quantitative PCR techniques. Our results revealed that the core gut microbiota, especially the fermentative bacteria were mainly sourced from the soil, but strongly stimulated with species-specificity, potential benefits for the host and soil health. The functional compositions of gut microbiota were altered by fertilization with fertilization duration being more influential than fertilization regimes. Moreover, the combination of organic and inorganic fertilization with the longer duration resulted in a higher richness and connectivity in the gut microbiota, and also their functional potential related to carbon (C), nitrogen, and phosphorus cycling, particularly the labile C decomposition, denitrification, and phosphate mobilization. We also found that long-term inorganic fertilization increased the abundance of pathogenic bacteria in the P. guillelmi gut. This study demonstrates that understanding earthworm gut microbiota can provide insights into how agricultural practices can potentially alter soil ecosystem functions through the interactions between soil and earthworm gut microbiotas. |
topic |
Organic-inorganic fertilization Earthworm species Gut microbiota Functional genes 16S rRNA |
url |
http://www.sciencedirect.com/science/article/pii/S0147651321007557 |
work_keys_str_mv |
AT qingfangbi howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT bingjiejin howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT dongzhu howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT yugenjiang howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT bangxiaozheng howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT patrickoconnor howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT xiaoruyang howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT andreasrichter howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT xianyonglin howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment AT yongguanzhu howcanfertilizationregimesanddurationsshapeearthwormgutmicrobiotainalongtermfieldexperiment |
_version_ |
1717814808821104640 |
spelling |
doaj-be755bc88c8e454e8c8575d9413338c42021-09-05T04:38:18ZengElsevierEcotoxicology and Environmental Safety0147-65132021-11-01224112643How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment?Qing-Fang Bi0Bing-Jie Jin1Dong Zhu2Yu-Gen Jiang3Bang-Xiao Zheng4Patrick O'Connor5Xiao-Ru Yang6Andreas Richter7Xian-Yong Lin8Yong-Guan Zhu9MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China; Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Division of Terrestrial Ecosystem Research, Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, AustriaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR ChinaFuyang Agricultural Technology Popularization Center, Hangzhou 311400, PR ChinaFaculty of Biological and Environmental Sciences, Ecosystems and Environment Research Programme, University of Helsinki, Lahti 15140, FinlandCentre for Global Food and Resources, University of Adelaide, Adelaide 5005, AustraliaKey Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR ChinaDivision of Terrestrial Ecosystem Research, Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, AustriaMOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China; Correspondence to: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China.MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China; Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR ChinaThe positive roles of earthworms on soil functionality has been extensively documented. The capacity of the earthworm gut microbiota on decomposition and nutrient cycling under long-term fertilization in field conditions has rarely been studied. Here, we report the structural, taxonomic, and functional responses of Eisenia foetida and Pheretima guillelmi gut microbiota to different fertilization regimes and durations using 16S rRNA gene-based Illumina sequencing and high-throughput quantitative PCR techniques. Our results revealed that the core gut microbiota, especially the fermentative bacteria were mainly sourced from the soil, but strongly stimulated with species-specificity, potential benefits for the host and soil health. The functional compositions of gut microbiota were altered by fertilization with fertilization duration being more influential than fertilization regimes. Moreover, the combination of organic and inorganic fertilization with the longer duration resulted in a higher richness and connectivity in the gut microbiota, and also their functional potential related to carbon (C), nitrogen, and phosphorus cycling, particularly the labile C decomposition, denitrification, and phosphate mobilization. We also found that long-term inorganic fertilization increased the abundance of pathogenic bacteria in the P. guillelmi gut. This study demonstrates that understanding earthworm gut microbiota can provide insights into how agricultural practices can potentially alter soil ecosystem functions through the interactions between soil and earthworm gut microbiotas.http://www.sciencedirect.com/science/article/pii/S0147651321007557Organic-inorganic fertilizationEarthworm speciesGut microbiotaFunctional genes16S rRNA |