Identification of genes differentially expressed in onion infected with <em>Iris yellow spot virus</em>

• Main Authors: Ahmed ABDELKHALEK, Adel ELMORSI, Omar ALSHEHABY, Neeti SANAN-MISHRA, Elsayed HAFEZ
• Format: Article
• Language: English
• Published: Firenze University Press 2018-09-01
• Series: Phytopathologia Mediterranea
• Subjects: DD-PCR; defence system; IYSV
• Online Access: https://oajournals.fupress.net/index.php/pm/article/view/5771

Iris yellow spot virus (IYSV) causes severe damage and economic losses in onion production. Differential display-PCR was used to study changes in the gene expression of IYSV-infected onion plants. Representative up-regulated and down-regulated genes were selected for further study. Based on sequence analysis, the up-regulated genes were identified as retrotransposon protein, disease resistance-like proteins, chitinase, pathogenesis-related protein, cytochrome oxidase, cytochrome c, pentatricopeptide repeat-containing protein and pectin methylesterase. A DNA-binding transcriptional repressor protein gene was greatly down-regulated. . Most of the identified genes are known to play essential roles in plant defence systems, and are newly identified in onion sequences.