Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p
Introduction and Objectives: Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) may be closely associated with Hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma (HCC). In this study, we investigated the expression and functions of a lncRNA, LINC0118...
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doaj-be8cb81ad00c454f9dccd02d366b20972021-06-17T04:46:16ZengElsevierAnnals of Hepatology1665-26812021-05-0122100269Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5pYing Yao0Fang Shu1Fang Wang2Xiaoqiang Wang3Zhengshe Guo4Haili Wang5Lu Li6Haigang Lv7Clinical laboratory, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054 Shaanxi, ChinaClinical laboratory, Third Hospital of Xi’an, 710000, Shaanxi, ChinaAnaesthesiology department, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054, Shaanxi, ChinaDepartment of Cancer Biology, City of Hope National Medical Center, Duarte, CA, 91010, USAAnaesthesiology department, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054, Shaanxi, ChinaAnaesthesiology department, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054, Shaanxi, ChinaAnaesthesiology department, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054, Shaanxi, ChinaAnaesthesiology department, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054, Shaanxi, China; Corresponding author.Introduction and Objectives: Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) may be closely associated with Hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma (HCC). In this study, we investigated the expression and functions of a lncRNA, LINC01189, in HCV-associated HCC. Patients or Materials and Methods: LINC01189 expression was measured in HCC tumors, HCV-infected HCC tumors and HCV-infected HCC cells. LINC01189 was overexpressed in HCV-infected HepG2 cells to measure its function on HCV-correlated cancer proliferation. In HCC cell lines of Huh7 and Hep3B, LINC01189 was upregulated to investigate its effects on cancer cell proliferation and 5-FU chemoresistance. The competing endogenous RNA (ceRNA) target of LINC01189, human microRNA-155-5p (hsa-miR-155-5p) was probed by dual-luciferase assay and qRT-PCR. Hsa-miR-155-5p was upregulated in LINC01189-overexpessed Huh7 and Hep3B cells to investigate their epigenetic correlation on HCC development regulation. Results: LINC01189 is downregulated in HCV-infected HCC tumors and cell lines. LINC01189 overexpression inhibited HCC cancer cell proliferation and 5-FU chemoresistance. Hsa-miR-155-5p was confirmed to be a ceRNA target of LINC01189 in HCC. Upregulating hsa-miR-155-5p reversed the LINC01189-mediated inhibition on HCC proliferation and 5-FU chemoresistance. Conclusions: LINC01189 downregulation is associated with HCV infection in HCC, and it has tumor-suppressing effects on HCC development through hsa-miR-155-5p.http://www.sciencedirect.com/science/article/pii/S1665268120301848HCVHCClncRNALINC01189hsa-miR-155-5p |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Yao Fang Shu Fang Wang Xiaoqiang Wang Zhengshe Guo Haili Wang Lu Li Haigang Lv |
spellingShingle |
Ying Yao Fang Shu Fang Wang Xiaoqiang Wang Zhengshe Guo Haili Wang Lu Li Haigang Lv Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p Annals of Hepatology HCV HCC lncRNA LINC01189 hsa-miR-155-5p |
author_facet |
Ying Yao Fang Shu Fang Wang Xiaoqiang Wang Zhengshe Guo Haili Wang Lu Li Haigang Lv |
author_sort |
Ying Yao |
title |
Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p |
title_short |
Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p |
title_full |
Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p |
title_fullStr |
Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p |
title_full_unstemmed |
Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p |
title_sort |
long noncoding rna linc01189 is associated with hcv-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-mir-155-5p |
publisher |
Elsevier |
series |
Annals of Hepatology |
issn |
1665-2681 |
publishDate |
2021-05-01 |
description |
Introduction and Objectives: Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) may be closely associated with Hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma (HCC). In this study, we investigated the expression and functions of a lncRNA, LINC01189, in HCV-associated HCC. Patients or Materials and Methods: LINC01189 expression was measured in HCC tumors, HCV-infected HCC tumors and HCV-infected HCC cells. LINC01189 was overexpressed in HCV-infected HepG2 cells to measure its function on HCV-correlated cancer proliferation. In HCC cell lines of Huh7 and Hep3B, LINC01189 was upregulated to investigate its effects on cancer cell proliferation and 5-FU chemoresistance. The competing endogenous RNA (ceRNA) target of LINC01189, human microRNA-155-5p (hsa-miR-155-5p) was probed by dual-luciferase assay and qRT-PCR. Hsa-miR-155-5p was upregulated in LINC01189-overexpessed Huh7 and Hep3B cells to investigate their epigenetic correlation on HCC development regulation. Results: LINC01189 is downregulated in HCV-infected HCC tumors and cell lines. LINC01189 overexpression inhibited HCC cancer cell proliferation and 5-FU chemoresistance. Hsa-miR-155-5p was confirmed to be a ceRNA target of LINC01189 in HCC. Upregulating hsa-miR-155-5p reversed the LINC01189-mediated inhibition on HCC proliferation and 5-FU chemoresistance. Conclusions: LINC01189 downregulation is associated with HCV infection in HCC, and it has tumor-suppressing effects on HCC development through hsa-miR-155-5p. |
topic |
HCV HCC lncRNA LINC01189 hsa-miR-155-5p |
url |
http://www.sciencedirect.com/science/article/pii/S1665268120301848 |
work_keys_str_mv |
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