Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity
Summary: Oncogenic KRAS mutations in hematopoietic stem cells cause RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD). KRAS plays essential roles in stemness maintenance in some types of stem cells. However, its roles in pluripotent stem cells (PSCs) are poorly understood. H...
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doaj-bec0e0806fec42e48f4d3ad662e1e2de2020-11-24T21:41:43ZengElsevierStem Cell Reports2213-67112018-08-01112380394Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation PropensityKenji Kubara0Kazuto Yamazaki1Yasuharu Ishihara2Takuya Naruto3Huan-Ting Lin4Ken Nishimura5Manami Ohtaka6Mahito Nakanishi7Masashi Ito8Kappei Tsukahara9Tomohiro Morio10Masatoshi Takagi11Makoto Otsu12Tsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan; Corresponding authorTsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan; Corresponding authorTsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, JapanDepartment of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, JapanDivision of Stem Cell Processing/Stem Cell Bank, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanLaboratory of Gene Regulation, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, JapanTOKIWA-Bio, Inc., 1-1-1 Higashi, Central 5, Tsukuba, Ibaraki 305-8565, JapanBiotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, JapanTsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, JapanTsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, JapanDepartment of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, JapanDepartment of Pediatrics and Developmental Biology, Graduate School of Medicine, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, JapanDivision of Stem Cell Processing/Stem Cell Bank, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, JapanSummary: Oncogenic KRAS mutations in hematopoietic stem cells cause RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD). KRAS plays essential roles in stemness maintenance in some types of stem cells. However, its roles in pluripotent stem cells (PSCs) are poorly understood. Here, we investigated the roles of KRAS on stemness in the context of induced PSCs (iPSCs). We used KRAS mutant (G13C/WT) and wild-type isogenic (WT/WT) iPSCs from the same RALD patients, as well as wild-type (WTed/WT) and heterozygous knockout (Δed/WT) iPSCs, both obtained by genome editing from the same G13C/WT clone. Compared with WT iPSCs, G13C/WT iPSCs displayed enforced retention of self-renewal and suppressed capacity for neuronal differentiation, while Δed/WT iPSCs showed normalized cellular characteristics similar to those of isogenic WTed/WT cells. The KRAS-ERK pathway, but not the KRAS-PI3K pathway, was shown to govern these G13C/WT-specific phenotypes, indicating the strong impact of the KRAS-ERK signaling upon self-renewal and differentiation propensity in human iPSCs. : In this article, Kubara, Yamazaki, and colleagues show that, using RALD patient-derived iPSCs, oncogenic KRAS activates mainly the MEK-ERK pathway, but not the PI3K-AKT pathway, leading to enforced retention of self-renewal and suppressed capacity for undergoing neuronal differentiation in human iPSCs. Keywords: iPSCs, KRAS, RAS-associated autoimmune lymphoproliferative syndrome-like disease, self-renewal, stemness, differentiation, MAPK pathwayhttp://www.sciencedirect.com/science/article/pii/S2213671118302716 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kenji Kubara Kazuto Yamazaki Yasuharu Ishihara Takuya Naruto Huan-Ting Lin Ken Nishimura Manami Ohtaka Mahito Nakanishi Masashi Ito Kappei Tsukahara Tomohiro Morio Masatoshi Takagi Makoto Otsu |
spellingShingle |
Kenji Kubara Kazuto Yamazaki Yasuharu Ishihara Takuya Naruto Huan-Ting Lin Ken Nishimura Manami Ohtaka Mahito Nakanishi Masashi Ito Kappei Tsukahara Tomohiro Morio Masatoshi Takagi Makoto Otsu Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity Stem Cell Reports |
author_facet |
Kenji Kubara Kazuto Yamazaki Yasuharu Ishihara Takuya Naruto Huan-Ting Lin Ken Nishimura Manami Ohtaka Mahito Nakanishi Masashi Ito Kappei Tsukahara Tomohiro Morio Masatoshi Takagi Makoto Otsu |
author_sort |
Kenji Kubara |
title |
Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity |
title_short |
Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity |
title_full |
Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity |
title_fullStr |
Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity |
title_full_unstemmed |
Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity |
title_sort |
status of kras in ipscs impacts upon self-renewal and differentiation propensity |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2018-08-01 |
description |
Summary: Oncogenic KRAS mutations in hematopoietic stem cells cause RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD). KRAS plays essential roles in stemness maintenance in some types of stem cells. However, its roles in pluripotent stem cells (PSCs) are poorly understood. Here, we investigated the roles of KRAS on stemness in the context of induced PSCs (iPSCs). We used KRAS mutant (G13C/WT) and wild-type isogenic (WT/WT) iPSCs from the same RALD patients, as well as wild-type (WTed/WT) and heterozygous knockout (Δed/WT) iPSCs, both obtained by genome editing from the same G13C/WT clone. Compared with WT iPSCs, G13C/WT iPSCs displayed enforced retention of self-renewal and suppressed capacity for neuronal differentiation, while Δed/WT iPSCs showed normalized cellular characteristics similar to those of isogenic WTed/WT cells. The KRAS-ERK pathway, but not the KRAS-PI3K pathway, was shown to govern these G13C/WT-specific phenotypes, indicating the strong impact of the KRAS-ERK signaling upon self-renewal and differentiation propensity in human iPSCs. : In this article, Kubara, Yamazaki, and colleagues show that, using RALD patient-derived iPSCs, oncogenic KRAS activates mainly the MEK-ERK pathway, but not the PI3K-AKT pathway, leading to enforced retention of self-renewal and suppressed capacity for undergoing neuronal differentiation in human iPSCs. Keywords: iPSCs, KRAS, RAS-associated autoimmune lymphoproliferative syndrome-like disease, self-renewal, stemness, differentiation, MAPK pathway |
url |
http://www.sciencedirect.com/science/article/pii/S2213671118302716 |
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