Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree
Abstract Mycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-04-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-87886-5 |
id |
doaj-bec0ee55fdce4e6da508acd89826a24d |
---|---|
record_format |
Article |
spelling |
doaj-bec0ee55fdce4e6da508acd89826a24d2021-04-25T11:32:19ZengNature Publishing GroupScientific Reports2045-23222021-04-0111111610.1038/s41598-021-87886-5Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak treeM. Sebastiana0A. Gargallo-Garriga1J. Sardans2M. Pérez-Trujillo3F. Monteiro4A. Figueiredo5M. Maia6R. Nascimento7M. Sousa Silva8A. N. Ferreira9C. Cordeiro10A. P. Marques11L. Sousa12R. Malhó13J. Peñuelas14Plant Functional Genomics Group, BioISI – Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de LisboaGlobal Change Research Institute of the Czech Academy of SciencesCREAFServei de Ressonàcia Magnètica Nuclear, Universitat Autònoma de BarcelonaCentre for Ecology, Evolution and Environmental Changes (CE3C), Faculdade de Ciências, Universidade de LisboaPlant Functional Genomics Group, BioISI – Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de LisboaPlant Functional Genomics Group, BioISI – Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de LisboaPlant Functional Genomics Group, BioISI – Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de LisboaLaboratório de FTICR e Espectrometria de Massa Estrutural, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de LisboaLaboratório de FTICR e Espectrometria de Massa Estrutural, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de LisboaLaboratório de FTICR e Espectrometria de Massa Estrutural, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de LisboaLaboratório de FTICR e Espectrometria de Massa Estrutural, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de LisboaCEAUL - Centro de Estatística e Aplicações, Faculdade de Ciências, Universidade de LisboaPlant Functional Genomics Group, BioISI – Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de LisboaCREAFAbstract Mycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the ectomycorrhizal fungus Pisolithus tinctorius were compared with non-colonized roots. Results show that compounds putatively corresponding to carbohydrates, organic acids, tannins, long-chain fatty acids and monoacylglycerols, were depleted in ectomycorrhizal cork oak colonized roots. Conversely, non-proteogenic amino acids, such as gamma-aminobutyric acid (GABA), and several putative defense-related compounds, including oxylipin-family compounds, terpenoids and B6 vitamers were induced in mycorrhizal roots. Transcriptomic analysis suggests the involvement of GABA in ectomycorrhizal symbiosis through increased synthesis and inhibition of degradation in mycorrhizal roots. Results from this global metabolomics analysis suggest decreases in root metabolites which are common components of exudates, and in compounds related to root external protective layers which could facilitate plant-fungal contact and enhance symbiosis. Root metabolic pathways involved in defense against stress were induced in ectomycorrhizal roots that could be involved in a plant mechanism to avoid uncontrolled growth of the fungal symbiont in the root apoplast. Several of the identified symbiosis-specific metabolites, such as GABA, may help to understand how ectomycorrhizal fungi such as P. tinctorius benefit their host plants.https://doi.org/10.1038/s41598-021-87886-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Sebastiana A. Gargallo-Garriga J. Sardans M. Pérez-Trujillo F. Monteiro A. Figueiredo M. Maia R. Nascimento M. Sousa Silva A. N. Ferreira C. Cordeiro A. P. Marques L. Sousa R. Malhó J. Peñuelas |
spellingShingle |
M. Sebastiana A. Gargallo-Garriga J. Sardans M. Pérez-Trujillo F. Monteiro A. Figueiredo M. Maia R. Nascimento M. Sousa Silva A. N. Ferreira C. Cordeiro A. P. Marques L. Sousa R. Malhó J. Peñuelas Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree Scientific Reports |
author_facet |
M. Sebastiana A. Gargallo-Garriga J. Sardans M. Pérez-Trujillo F. Monteiro A. Figueiredo M. Maia R. Nascimento M. Sousa Silva A. N. Ferreira C. Cordeiro A. P. Marques L. Sousa R. Malhó J. Peñuelas |
author_sort |
M. Sebastiana |
title |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
title_short |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
title_full |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
title_fullStr |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
title_full_unstemmed |
Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
title_sort |
metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-04-01 |
description |
Abstract Mycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the ectomycorrhizal fungus Pisolithus tinctorius were compared with non-colonized roots. Results show that compounds putatively corresponding to carbohydrates, organic acids, tannins, long-chain fatty acids and monoacylglycerols, were depleted in ectomycorrhizal cork oak colonized roots. Conversely, non-proteogenic amino acids, such as gamma-aminobutyric acid (GABA), and several putative defense-related compounds, including oxylipin-family compounds, terpenoids and B6 vitamers were induced in mycorrhizal roots. Transcriptomic analysis suggests the involvement of GABA in ectomycorrhizal symbiosis through increased synthesis and inhibition of degradation in mycorrhizal roots. Results from this global metabolomics analysis suggest decreases in root metabolites which are common components of exudates, and in compounds related to root external protective layers which could facilitate plant-fungal contact and enhance symbiosis. Root metabolic pathways involved in defense against stress were induced in ectomycorrhizal roots that could be involved in a plant mechanism to avoid uncontrolled growth of the fungal symbiont in the root apoplast. Several of the identified symbiosis-specific metabolites, such as GABA, may help to understand how ectomycorrhizal fungi such as P. tinctorius benefit their host plants. |
url |
https://doi.org/10.1038/s41598-021-87886-5 |
work_keys_str_mv |
AT msebastiana metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT agargallogarriga metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT jsardans metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT mpereztrujillo metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT fmonteiro metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT afigueiredo metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT mmaia metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT rnascimento metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT msousasilva metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT anferreira metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT ccordeiro metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT apmarques metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT lsousa metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT rmalho metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree AT jpenuelas metabolomicsandtranscriptomicstodeciphermolecularmechanismsunderlyingectomycorrhizalrootcolonizationofanoaktree |
_version_ |
1721509666565914624 |