A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II.
Two methods are compared for measuring the kinetic parameters of apolipoprotein A-I and A-II metabolism in human plasma. In the first, high density lipoprotein apoproteins were radioiodinated in situ in the lipoprotein particle (endogenous apoprotein labeling) while in the second, individually label...
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1978-07-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520412994 |
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doaj-bec0fc3e2dfd44d784f57e6c9e05fab52021-04-24T05:53:30ZengElsevierJournal of Lipid Research0022-22751978-07-01195656661A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II.J ShepherdC J PackardA M Gotto, JrO D TauntonTwo methods are compared for measuring the kinetic parameters of apolipoprotein A-I and A-II metabolism in human plasma. In the first, high density lipoprotein apoproteins were radioiodinated in situ in the lipoprotein particle (endogenous apoprotein labeling) while in the second, individually labeled apolipoprotein A-I or A-II was incorporated into the particle by in vitro incubation (exogenous apoprotein labeling). The catabolic clearance rate of exogenously labeled apolipoprotein A-I was consistently faster than that of endogenous apolipoprotein A-I. Conversely, endogenously and exogenously labeled apolipoprotein A-II were catabolized at identical rates. The fractional plasma clearance rates of endogenous apolipoproteins A-I and A-II were the same.http://www.sciencedirect.com/science/article/pii/S0022227520412994 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J Shepherd C J Packard A M Gotto, Jr O D Taunton |
spellingShingle |
J Shepherd C J Packard A M Gotto, Jr O D Taunton A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II. Journal of Lipid Research |
author_facet |
J Shepherd C J Packard A M Gotto, Jr O D Taunton |
author_sort |
J Shepherd |
title |
A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II. |
title_short |
A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II. |
title_full |
A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II. |
title_fullStr |
A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II. |
title_full_unstemmed |
A comparison of two methods to investigate the metabolism of human apolipoproteins A-I and and A-II. |
title_sort |
comparison of two methods to investigate the metabolism of human apolipoproteins a-i and and a-ii. |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1978-07-01 |
description |
Two methods are compared for measuring the kinetic parameters of apolipoprotein A-I and A-II metabolism in human plasma. In the first, high density lipoprotein apoproteins were radioiodinated in situ in the lipoprotein particle (endogenous apoprotein labeling) while in the second, individually labeled apolipoprotein A-I or A-II was incorporated into the particle by in vitro incubation (exogenous apoprotein labeling). The catabolic clearance rate of exogenously labeled apolipoprotein A-I was consistently faster than that of endogenous apolipoprotein A-I. Conversely, endogenously and exogenously labeled apolipoprotein A-II were catabolized at identical rates. The fractional plasma clearance rates of endogenous apolipoproteins A-I and A-II were the same. |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520412994 |
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