CO-releasing molecules (CORM-2)-liberated CO attenuates leukocytes infiltration in the renal tissue of thermally injured mice

• Main Author: Bingwei Sun, Zhiwei Sun, Qin Jin, Xi Chen
• Format: Article
• Language: English
• Published: Ivyspring International Publisher 2008-01-01
• Series: International Journal of Biological Sciences
• Online Access: http://www.biolsci.org/v04p0176.htm

<p><i>Objective</i><b>:</b> To determine whether the CO-releasing molecule -liberated CO attenuates infiltration of leukocytes in the renal tissue of thermally injured mice.</p> <p><i>Materials and methods</i><b>:</b> Twenty-eight mice were assigned to four groups. Mice in sham group (<i>n</i>=7) were underwent sham thermal injury, whereas mice in burn group (<i>n</i>=7) received 15% total body surface area (TBSA) full-thickness thermal injury. Mice in burn+CORM-2 group (<i>n</i>=7) underwent thermal injury followed by immediate administration of CORM-2 (8mg/kg, i.v.), whereas mice in burn+iCORM-2 group (<i>n</i>=7) underwent thermal injury followed by administration of iCORM-2 (an inactive compound used as negative control). Histological alterations and granulocytes infiltration in kidney were assessed alongised PMN accumulation, activation of NF-&#312;&#914;, expressions of ICAM-1 and HO-1 expression in renal tissues.</p> <p><i>Results</i><b>: </b>Treatment of thermally injured mice with CORM-2 significantly attenuated PMN accumulation and prevented activation of NF-&#312;&#914; in the kidney. This was accompanied by a decrease of the expression of ICAM-1 and an increase in HO-1 expression. In parallel, burn-induced granulocytes infiltration in renal tissue was markedly decreased by treatment with CORM-2.</p> <p><i>Conclusions</i><b>: </b>CO delivered by CORM-2 attenuates leukocytes infiltration in the kidney of burned mice by interfering with NF-&#312;&#914; activation, protein expression of ICAM-1 and therefore suppressing endothelial cells pro-adhesive phenotype.</p>