Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixtures

Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixtures

Abstract Background Trypanosomatids are a group of protozoan parasites that includes the etiologic agents of important human illnesses as Chagas disease, sleeping sickness and leishmaniasis. These parasites have a significant distinction from other eukaryotes concerning mRNA structure, since all mat...

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Bibliographic Details
Main Authors: Rafael Luis Kessler, Daniela Parada Pavoni, Marco Aurelio Krieger, Christian Macagnan Probst
Format: Article
Language:English
Published: BMC 2017-10-01
Series:BMC Genomics
Subjects:
Trypanosoma cruzi
Trypanosomatid
RNA-Seq
Transcriptomics
RNA amplification
T7 RNA polymerase
Online Access:http://link.springer.com/article/10.1186/s12864-017-4163-y

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http://link.springer.com/article/10.1186/s12864-017-4163-y

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