Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization

Summary: Staphylococcus aureus is a leading cause of skin and soft issue infection, but paradoxically, it also transiently, and often harmlessly, colonizes human skin. An obstacle to understanding this contradiction has been a shortage of in vivo models reproducing the unique structure and immunolog...

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Main Authors: Anette Schulz, Long Jiang, Lisanne de Vor, Marcus Ehrström, Fredrik Wermeling, Liv Eidsmo, Keira Melican
Format: Article
Language:English
Published: Elsevier 2019-10-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124719312434
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spelling doaj-bed1c0a845fc4ec8b22626a4f785fd532020-11-25T02:20:07ZengElsevierCell Reports2211-12472019-10-0129510741081.e5Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient ColonizationAnette Schulz0Long Jiang1Lisanne de Vor2Marcus Ehrström3Fredrik Wermeling4Liv Eidsmo5Keira Melican6Swedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institute, Stockholm 171 77, SwedenDivision of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm 171 77, SwedenSwedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institute, Stockholm 171 77, SwedenDepartment of Reconstructive Plastic Surgery, Karolinska University Hospital Solna, Stockholm 171 77, SwedenDivision of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm 171 77, SwedenDivision of Rheumatology, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm 171 77, SwedenSwedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institute, Stockholm 171 77, Sweden; Corresponding authorSummary: Staphylococcus aureus is a leading cause of skin and soft issue infection, but paradoxically, it also transiently, and often harmlessly, colonizes human skin. An obstacle to understanding this contradiction has been a shortage of in vivo models reproducing the unique structure and immunology of human skin. In this work, we developed a humanized model to study how healthy adult human skin responds to colonizing methicillin-resistant S. aureus (MRSA). We demonstrate the importance of the outer stratum corneum as the major site of bacterial colonization and how noninvasive MRSA adhesion to corneocytes induces a local inflammatory response in underlying skin layers. This signaling recruits neutrophils to the skin, where they control bacterial numbers, mediating transiency in colonization. This work highlights the spatiotemporal aspects of human skin colonization and demonstrates a subclinical inflammatory response to noninvasive MRSA that allows human skin to regulate the bacterial population at its outer surface. : Using a xenograft humanized model, Schulz et al. show how noninvasive colonization of the human skin corneal layer by methicillin-resistant Staphylococcus aureus (MRSA) induces a local tissue response. This response differs from the previously reported mouse models. MRSA colonization induces neutrophil recruitment to the skin, which facilitates transiency. Keywords: Staphylococcus aureus, MRSA, skin microbiota, xenograft, humanized, in vivo, neutrophils, stratum corneum, colonisationhttp://www.sciencedirect.com/science/article/pii/S2211124719312434
collection DOAJ
language English
format Article
sources DOAJ
author Anette Schulz
Long Jiang
Lisanne de Vor
Marcus Ehrström
Fredrik Wermeling
Liv Eidsmo
Keira Melican
spellingShingle Anette Schulz
Long Jiang
Lisanne de Vor
Marcus Ehrström
Fredrik Wermeling
Liv Eidsmo
Keira Melican
Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
Cell Reports
author_facet Anette Schulz
Long Jiang
Lisanne de Vor
Marcus Ehrström
Fredrik Wermeling
Liv Eidsmo
Keira Melican
author_sort Anette Schulz
title Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
title_short Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
title_full Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
title_fullStr Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
title_full_unstemmed Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
title_sort neutrophil recruitment to noninvasive mrsa at the stratum corneum of human skin mediates transient colonization
publisher Elsevier
series Cell Reports
issn 2211-1247
publishDate 2019-10-01
description Summary: Staphylococcus aureus is a leading cause of skin and soft issue infection, but paradoxically, it also transiently, and often harmlessly, colonizes human skin. An obstacle to understanding this contradiction has been a shortage of in vivo models reproducing the unique structure and immunology of human skin. In this work, we developed a humanized model to study how healthy adult human skin responds to colonizing methicillin-resistant S. aureus (MRSA). We demonstrate the importance of the outer stratum corneum as the major site of bacterial colonization and how noninvasive MRSA adhesion to corneocytes induces a local inflammatory response in underlying skin layers. This signaling recruits neutrophils to the skin, where they control bacterial numbers, mediating transiency in colonization. This work highlights the spatiotemporal aspects of human skin colonization and demonstrates a subclinical inflammatory response to noninvasive MRSA that allows human skin to regulate the bacterial population at its outer surface. : Using a xenograft humanized model, Schulz et al. show how noninvasive colonization of the human skin corneal layer by methicillin-resistant Staphylococcus aureus (MRSA) induces a local tissue response. This response differs from the previously reported mouse models. MRSA colonization induces neutrophil recruitment to the skin, which facilitates transiency. Keywords: Staphylococcus aureus, MRSA, skin microbiota, xenograft, humanized, in vivo, neutrophils, stratum corneum, colonisation
url http://www.sciencedirect.com/science/article/pii/S2211124719312434
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