Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla.
To analyze the expression of transforming growth factor-ß1 in heterotopic grafts of adult dental apical papilla. Methodology: The apical papilla of adult Wistar rats was grafted in the ear of the same donor rats 1, 3, 7 and 14 days after grafting, rats were perfused and the tissue containing the gra...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Universidad de Concepción.
2015-02-01
|
Series: | Journal of Oral Research |
Subjects: | |
Online Access: | http://www.joralres.com/index.php/JOR/article/view/joralres.2015.009/135 |
id |
doaj-bed1f16d6a294a2cabbdb95b17c4973d |
---|---|
record_format |
Article |
spelling |
doaj-bed1f16d6a294a2cabbdb95b17c4973d2020-11-25T02:17:45ZengUniversidad de Concepción.Journal of Oral Research0719-24600719-24792015-02-0141384310.17126/joralres.2015.009Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla.Telmo Mezadri0David Tames1Xana Ortolan2José Armengol3Centro de Ciencias de la Salud. Universidade do Vale do Itajaí. SC, Brasil.Centro de Ciencias de la Salud. Universidade do Vale do Itajaí. SC, Brasil.Centro de Ciencias de la Salud. Universidade do Vale do Itajaí. SC, Brasil.Departamento de Fisiología, Anatomía y Biología Celular. Universidad Pablo de Olavide. Sevilla, España.To analyze the expression of transforming growth factor-ß1 in heterotopic grafts of adult dental apical papilla. Methodology: The apical papilla of adult Wistar rats was grafted in the ear of the same donor rats 1, 3, 7 and 14 days after grafting, rats were perfused and the tissue containing the graft was processed for histological conventional technique and for immunohistochemical detection of transforming growth factor-ß1. Results: Heterotopically grafted apical papilla developed osteoid dentine. In an early post-grafting stage, odontoblast-like cells organized themselves in palisade and synthesized dentine. However, newly formed dentine possessed the structural appearance of reactive osteoid dentine, which was systematically destroyed by the activity of osteoclaste-like cells. Transforming Growth Factor-ß1 was observed in mesenchymal cells, extracellular matrix of the graft and surrounding host tissue, while odontoblast-like cells were systematically devoid of immunoreactivity. Conclusion: The different expression of transforming growth factor-ß1 between normal tissue and grafted tissue development suggests that in heterotopic graft conditions the inflammatory mediation of the transforming growth factor-ß1 prevails against its morphogenetic rolehttp://www.joralres.com/index.php/JOR/article/view/joralres.2015.009/135OdontoblastApical papillaTGF-ß1Graft. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Telmo Mezadri David Tames Xana Ortolan José Armengol |
spellingShingle |
Telmo Mezadri David Tames Xana Ortolan José Armengol Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. Journal of Oral Research Odontoblast Apical papilla TGF-ß1 Graft. |
author_facet |
Telmo Mezadri David Tames Xana Ortolan José Armengol |
author_sort |
Telmo Mezadri |
title |
Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. |
title_short |
Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. |
title_full |
Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. |
title_fullStr |
Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. |
title_full_unstemmed |
Transforming Growth Factor -ß1 (TGF-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. |
title_sort |
transforming growth factor -ß1 (tgf-ß1) immunoreactivity in heterotopic grafts of adult dental apical papilla. |
publisher |
Universidad de Concepción. |
series |
Journal of Oral Research |
issn |
0719-2460 0719-2479 |
publishDate |
2015-02-01 |
description |
To analyze the expression of transforming growth factor-ß1 in heterotopic grafts of adult dental apical papilla. Methodology: The apical papilla of adult Wistar rats was grafted in the ear of the same donor rats 1, 3, 7 and 14 days after grafting, rats were perfused and the tissue containing the graft was processed for histological conventional technique and for immunohistochemical detection of transforming growth factor-ß1. Results: Heterotopically grafted apical papilla developed osteoid dentine. In an early post-grafting stage, odontoblast-like cells organized themselves in palisade and synthesized dentine. However, newly formed dentine possessed the structural appearance of reactive osteoid dentine, which was systematically destroyed by the activity of osteoclaste-like cells. Transforming Growth Factor-ß1 was observed in mesenchymal cells, extracellular matrix of the graft and surrounding host tissue, while odontoblast-like cells were systematically devoid of immunoreactivity. Conclusion: The different expression of transforming growth factor-ß1 between normal tissue and grafted tissue development suggests that in heterotopic graft conditions the inflammatory mediation of the transforming growth factor-ß1 prevails against its morphogenetic role |
topic |
Odontoblast Apical papilla TGF-ß1 Graft. |
url |
http://www.joralres.com/index.php/JOR/article/view/joralres.2015.009/135 |
work_keys_str_mv |
AT telmomezadri transforminggrowthfactorß1tgfß1immunoreactivityinheterotopicgraftsofadultdentalapicalpapilla AT davidtames transforminggrowthfactorß1tgfß1immunoreactivityinheterotopicgraftsofadultdentalapicalpapilla AT xanaortolan transforminggrowthfactorß1tgfß1immunoreactivityinheterotopicgraftsofadultdentalapicalpapilla AT josearmengol transforminggrowthfactorß1tgfß1immunoreactivityinheterotopicgraftsofadultdentalapicalpapilla |
_version_ |
1724885265283022848 |