Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors
Transaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, their stability is still a concern for widespread application. One of the reasons for their instability is the need to use an excess of the amino donor when trying to synthesise amines w...
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doaj-bf025e57cd164c499f1a311e6880e3f32021-04-20T23:04:36ZengMDPI AGCatalysts2073-43442021-04-011152052010.3390/catal11040520Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane ReactorsDavid Roura Padrosa0Zoya Nissar1Francesca Paradisi2Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, SwitzerlandSchool of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, UKDepartment of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, SwitzerlandTransaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, their stability is still a concern for widespread application. One of the reasons for their instability is the need to use an excess of the amino donor when trying to synthesise amines with unfavourable equilibria. To circumvent this, recycling systems for the amino donor, such as amino acid dehydrogenases or aldolases, have proved useful to push the equilibria while avoiding high amino donor concentrations. In this work, we report the use of a new alanine dehydrogenase from the halotolerant bacteria <i>Halomonas elongata</i> which exhibits excellent stability to different cosolvents, combined with the well characterised CbFDH as a recycling system of L-alanine for the amination of three model substrates with unfavourable equilibria. In a step forward, the amino donor recycling system has been co-immobilised and used in flow with success as well as re-used as a dialysis enclosed system for the amination of an aromatic aldehyde.https://www.mdpi.com/2073-4344/11/4/520Enzyme immobilizationtransaminaseamino acid dehydrogenaseflow biocatalysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David Roura Padrosa Zoya Nissar Francesca Paradisi |
spellingShingle |
David Roura Padrosa Zoya Nissar Francesca Paradisi Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors Catalysts Enzyme immobilization transaminase amino acid dehydrogenase flow biocatalysis |
author_facet |
David Roura Padrosa Zoya Nissar Francesca Paradisi |
author_sort |
David Roura Padrosa |
title |
Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors |
title_short |
Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors |
title_full |
Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors |
title_fullStr |
Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors |
title_full_unstemmed |
Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors |
title_sort |
efficient amino donor recycling in amination reactions: development of a new alanine dehydrogenase in continuous flow and dialysis membrane reactors |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2021-04-01 |
description |
Transaminases have arisen as one of the main biocatalysts for amine production but despite their many advantages, their stability is still a concern for widespread application. One of the reasons for their instability is the need to use an excess of the amino donor when trying to synthesise amines with unfavourable equilibria. To circumvent this, recycling systems for the amino donor, such as amino acid dehydrogenases or aldolases, have proved useful to push the equilibria while avoiding high amino donor concentrations. In this work, we report the use of a new alanine dehydrogenase from the halotolerant bacteria <i>Halomonas elongata</i> which exhibits excellent stability to different cosolvents, combined with the well characterised CbFDH as a recycling system of L-alanine for the amination of three model substrates with unfavourable equilibria. In a step forward, the amino donor recycling system has been co-immobilised and used in flow with success as well as re-used as a dialysis enclosed system for the amination of an aromatic aldehyde. |
topic |
Enzyme immobilization transaminase amino acid dehydrogenase flow biocatalysis |
url |
https://www.mdpi.com/2073-4344/11/4/520 |
work_keys_str_mv |
AT davidrourapadrosa efficientaminodonorrecyclinginaminationreactionsdevelopmentofanewalaninedehydrogenaseincontinuousflowanddialysismembranereactors AT zoyanissar efficientaminodonorrecyclinginaminationreactionsdevelopmentofanewalaninedehydrogenaseincontinuousflowanddialysismembranereactors AT francescaparadisi efficientaminodonorrecyclinginaminationreactionsdevelopmentofanewalaninedehydrogenaseincontinuousflowanddialysismembranereactors |
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1721517181969104896 |