Functional Analysis of Three Rice Chloroplast Transit Peptides

Functional Analysis of Three Rice Chloroplast Transit Peptides

Chloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chl...

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Main Authors: Lei He, Guang Chen, Sen Zhang, Zhennan Qiu, Jiang Hu, Dali Zeng, Guangheng Zhang, Guojun Dong, Zhenyu Gao, Deyong Ren, Lan Shen, Longbiao Guo, Qian Qian, Li Zhu
Format: Article
Language:English
Published: Elsevier 2019-01-01
Series:Rice Science
Online Access:http://www.sciencedirect.com/science/article/pii/S1672630818300830
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spelling doaj-bf164f4eba504ddc918edae8bf1a4ae22020-11-24T21:44:29ZengElsevierRice Science1672-63082019-01-012611120Functional Analysis of Three Rice Chloroplast Transit PeptidesLei He0Guang Chen1Sen Zhang2Zhennan Qiu3Jiang Hu4Dali Zeng5Guangheng Zhang6Guojun Dong7Zhenyu Gao8Deyong Ren9Lan Shen10Longbiao Guo11Qian Qian12Li Zhu13State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaCorresponding authors.; State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaCorresponding authors.; State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, ChinaChloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chloroplasts. Fusion proteins lacking the CTP remained located in the cytoplasm. Furthermore, we constructed green fluorescent protein fusion vectors with the three CTPs and three non-chloroplast-localized proteins, Ghd10, MULTI-FLORET SPIKELET1 (MFS1), and SHORTENED UPPERMOST INTERNODE 1 (SUI1). After transforming these constructs into rice protoplasts, the fusion proteins all localized in the chloroplasts. Collectively, our results showed that these CTPs can transport non-chloroplast-localized proteins into the chloroplasts, and more importantly, these CTPs can be applied to engineer chloroplast metabolism. Keywords: rice, chloroplast transit peptide, translocated function, subcellular locationhttp://www.sciencedirect.com/science/article/pii/S1672630818300830
collection DOAJ
language English
format Article
sources DOAJ
author Lei He
Guang Chen
Sen Zhang
Zhennan Qiu
Jiang Hu
Dali Zeng
Guangheng Zhang
Guojun Dong
Zhenyu Gao
Deyong Ren
Lan Shen
Longbiao Guo
Qian Qian
Li Zhu
spellingShingle Lei He
Guang Chen
Sen Zhang
Zhennan Qiu
Jiang Hu
Dali Zeng
Guangheng Zhang
Guojun Dong
Zhenyu Gao
Deyong Ren
Lan Shen
Longbiao Guo
Qian Qian
Li Zhu
Functional Analysis of Three Rice Chloroplast Transit Peptides
Rice Science
author_facet Lei He
Guang Chen
Sen Zhang
Zhennan Qiu
Jiang Hu
Dali Zeng
Guangheng Zhang
Guojun Dong
Zhenyu Gao
Deyong Ren
Lan Shen
Longbiao Guo
Qian Qian
Li Zhu
author_sort Lei He
title Functional Analysis of Three Rice Chloroplast Transit Peptides
title_short Functional Analysis of Three Rice Chloroplast Transit Peptides
title_full Functional Analysis of Three Rice Chloroplast Transit Peptides
title_fullStr Functional Analysis of Three Rice Chloroplast Transit Peptides
title_full_unstemmed Functional Analysis of Three Rice Chloroplast Transit Peptides
title_sort functional analysis of three rice chloroplast transit peptides
publisher Elsevier
series Rice Science
issn 1672-6308
publishDate 2019-01-01
description Chloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chloroplasts. Fusion proteins lacking the CTP remained located in the cytoplasm. Furthermore, we constructed green fluorescent protein fusion vectors with the three CTPs and three non-chloroplast-localized proteins, Ghd10, MULTI-FLORET SPIKELET1 (MFS1), and SHORTENED UPPERMOST INTERNODE 1 (SUI1). After transforming these constructs into rice protoplasts, the fusion proteins all localized in the chloroplasts. Collectively, our results showed that these CTPs can transport non-chloroplast-localized proteins into the chloroplasts, and more importantly, these CTPs can be applied to engineer chloroplast metabolism. Keywords: rice, chloroplast transit peptide, translocated function, subcellular location
url http://www.sciencedirect.com/science/article/pii/S1672630818300830
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