Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities
Background: Ginseng extracts and ginseng-fermented products are widely used as functional cosmetic ingredients for their whitening and antiwrinkle effects. Recently, increasing attention has been given to bioactive metabolites isolated from endophytic fungi. However, little is known about the bioact...
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doaj-bf16e70e74cb4a21bf116b0526f92bdf2021-01-02T05:07:53ZengElsevierJournal of Ginseng Research1226-84532021-01-0145198107Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activitiesKyuri Kim0Hae-In Jeong1Inho Yang2Sang-Jip Nam3Kyung-Min Lim4College of Pharmacy, Ewha Womans University, Seoul, Republic of KoreaDepartment of Chemistry and Nanoscience, Global Top 5 Program, Ewha Womans University, Seoul, Republic of KoreaDepartment of Convergence Study on the Ocean Science and Technology, Korea Maritime and Ocean University, Busan, Republic of KoreaDepartment of Chemistry and Nanoscience, Global Top 5 Program, Ewha Womans University, Seoul, Republic of Korea; Corresponding author. Department of Chemistry and Nanoscience, Global Top 5 Program, Ewha Womans University, Seoul, 03760, Republic of Korea.College of Pharmacy, Ewha Womans University, Seoul, Republic of Korea; Corresponding author. College of Pharmacy, Ewha Womans University, Seoul, 03760, Republic of Korea.Background: Ginseng extracts and ginseng-fermented products are widely used as functional cosmetic ingredients for their whitening and antiwrinkle effects. Recently, increasing attention has been given to bioactive metabolites isolated from endophytic fungi. However, little is known about the bioactive metabolites of the fungi associated with Panax ginseng Meyer. Methods: An endophytic fungus, Penicillium sp. SNF123 was isolated from the root of P. ginseng, from which acremonidin E was purified. Acremonidin E was tested on melanin synthesis in the murine melanoma cell line B16F10, in the human melanoma cell line MNT-1, and in a pigmented 3D-human skin model, Melanoderm. Results: Acremonidin E reduced melanogenesis in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 cells with minimal cytotoxicity. qRT–PCR analysis demonstrated that acremonidin E downregulated melanogenic genes, including tyrosinase and tyrosinase-related protein 1 (TRP-1), while their enzymatic activities were unaffected. The antimelanogenic effects of acremonidin E were further confirmed in MNT-1 and a pigmented 3D human epidermal skin model, Melanoderm. Immunohistological examination of the Melanoderm further confirmed the regression of both melanin synthesis and melanocyte activation in the treated tissue. Conclusion: This study demonstrates that acremonidin E, a bioactive metabolite derived from a fungal endophyte of P. ginseng, can inhibit melanin synthesis by downregulating tyrosinase, illuminating the potential utility of microorganisms associated with P. ginseng for cosmetic ingredients.http://www.sciencedirect.com/science/article/pii/S1226845319302581Acremonidin EEndophytic fungusMelanogenesisPanax ginsengPenicillium sp. SNF12 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyuri Kim Hae-In Jeong Inho Yang Sang-Jip Nam Kyung-Min Lim |
spellingShingle |
Kyuri Kim Hae-In Jeong Inho Yang Sang-Jip Nam Kyung-Min Lim Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities Journal of Ginseng Research Acremonidin E Endophytic fungus Melanogenesis Panax ginseng Penicillium sp. SNF12 |
author_facet |
Kyuri Kim Hae-In Jeong Inho Yang Sang-Jip Nam Kyung-Min Lim |
author_sort |
Kyuri Kim |
title |
Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities |
title_short |
Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities |
title_full |
Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities |
title_fullStr |
Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities |
title_full_unstemmed |
Acremonidin E produced by Penicillium sp. SNF123, a fungal endophyte of Panax ginseng, has antimelanogenic activities |
title_sort |
acremonidin e produced by penicillium sp. snf123, a fungal endophyte of panax ginseng, has antimelanogenic activities |
publisher |
Elsevier |
series |
Journal of Ginseng Research |
issn |
1226-8453 |
publishDate |
2021-01-01 |
description |
Background: Ginseng extracts and ginseng-fermented products are widely used as functional cosmetic ingredients for their whitening and antiwrinkle effects. Recently, increasing attention has been given to bioactive metabolites isolated from endophytic fungi. However, little is known about the bioactive metabolites of the fungi associated with Panax ginseng Meyer. Methods: An endophytic fungus, Penicillium sp. SNF123 was isolated from the root of P. ginseng, from which acremonidin E was purified. Acremonidin E was tested on melanin synthesis in the murine melanoma cell line B16F10, in the human melanoma cell line MNT-1, and in a pigmented 3D-human skin model, Melanoderm. Results: Acremonidin E reduced melanogenesis in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 cells with minimal cytotoxicity. qRT–PCR analysis demonstrated that acremonidin E downregulated melanogenic genes, including tyrosinase and tyrosinase-related protein 1 (TRP-1), while their enzymatic activities were unaffected. The antimelanogenic effects of acremonidin E were further confirmed in MNT-1 and a pigmented 3D human epidermal skin model, Melanoderm. Immunohistological examination of the Melanoderm further confirmed the regression of both melanin synthesis and melanocyte activation in the treated tissue. Conclusion: This study demonstrates that acremonidin E, a bioactive metabolite derived from a fungal endophyte of P. ginseng, can inhibit melanin synthesis by downregulating tyrosinase, illuminating the potential utility of microorganisms associated with P. ginseng for cosmetic ingredients. |
topic |
Acremonidin E Endophytic fungus Melanogenesis Panax ginseng Penicillium sp. SNF12 |
url |
http://www.sciencedirect.com/science/article/pii/S1226845319302581 |
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