Development of molecularly imprinted biomimetic immunoassay method based on quantum dot marker for detection of phthalates

• Main Authors: Ximo Wang, Chen Chen, Longhua Xu, Hongyan Zhang, Zhixiang Xu
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2019-01-01
• Series: Food and Agricultural Immunology
• Subjects: biomimetic immunoassay; molecular imprinting; quantum dots; dibutyl phthalate
• Online Access: http://dx.doi.org/10.1080/09540105.2019.1649371
• ISSN: 0954-0105; 1465-3443

In this study, a direct competitive molecularly imprinted biomimetic immunoassay method was developed for determination of the plasticizer dibutyl phthalate. Water-soluble carboxyl quantum dots were used as the marker. A hydrophilic molecularly imprinted polymer synthesized directly on the surface of a 96-well plate was used as biomimetic antibody. Under the optimal conditions, the limit of detection and the sensitivity of the method were 0.011 and 0.136 mg/L respectively. The cross-reaction values of two structural analogs were 4.75% and 6.89%, this indicaties that the method has the good specificity. Edible oil samples spiked with dibutyl phthalate were assayed, and good recoveries ranging from 80.0% to 111.5% were obtained. The method was used to detect dibutyl phthalate residues in liquors, and there were no significant differences obtained using the developed method and those obtained using gas chromatography.